Trypanosoma cruzi:: Analysis of the complete PUF RNA-binding protein family
Trypanosoma cruzi:: Analysis of the complete PUF RNA-binding protein family
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DOI：
10.1016/j.exppara.2005.12.015
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发表时间：
2006-06-01
影响因子：
2.1
通讯作者：
Vazquez, Martin P.
中科院分区：
文献类型：
--
作者：
Caro, Florence;Bercovich, Natalia;Vazquez, Martin P.
PUF家族中的RNA结合蛋白通过与真核生物中的3‘非编码区序列元件结合来调控mRNAs的命运。在锥体中，其基因表达是多顺反子的，并且几乎完全由转录后过程控制，PUF蛋白可能发挥关键作用。我们报道了由10个成员组成的克氏锥虫PUF蛋白家族的完整分析。在计算机分析中，根据推测的结合特异性，预测在克鲁兹毛滴虫家族中至少存在三个主要群体。利用酵母三杂交试验，我们检验了TcPUF1、TcPUF3、TcPUF5和TcPUF8作为这些群体的代表的一些预测。对克氏锥虫基因组的数据挖掘使我们描述了最保守的群体TcPUF1和TcPUF2的TcPUF的假定结合靶点。这些目标包括线粒体蛋白和蛋白激酶的基因。最后，免疫定位实验表明，TcPUF1定位于细胞质中的多个离散区域，支持其所提出的功能。(C)2005 Elsevier Inc.保留所有权利。
The members of the PUF family of RNA-binding proteins regulate the fate of mRNAs by binding to their 3'UTR sequence elements in eukaryotes. In trypanosomes, for which gene expression is polycistronic and controlled almost exclusively by post-transcriptional processes, PUF proteins could play a crucial role. We report here the complete analysis of the PUF protein family of Trypanosoma cruzi composed of 10 members. In silico analysis predicts the existence of at least three major groups within the T. cruzi family, based on their putative binding specificity. Using yeast three hybrid assays, we tested some of these predictions for TcPUF1, TcPUF3, TcPUF5, and TcPUF8 as representatives of these groups. Data mining of the T. cruzi genome led us to describe putative binding targets for the TcPUFs of the most conserved group, TcPUF1 and TcPUF2. The targets include genes for mitochondrial proteins and protein kinases. Finally, immunolocalization experiments showed that TcPUF1 is localized in multiple discrete foci in the cytoplasm supporting its proposed function. (c) 2005 Elsevier Inc. All rights reserved.