ORGANIC GROWTH FACTOR REQUIREMENTS OF TOBACCO TISSUE CULTURES
ORGANIC GROWTH FACTOR REQUIREMENTS OF TOBACCO TISSUE CULTURES
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DOI：
10.1111/j.1399-3054.1965.tb06874.x
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发表时间：
1965-01-01
影响因子：
6.4
通讯作者：
SKOOG, F
中科院分区：
文献类型：
--
作者：
LINSMAIER, EM;SKOOG, F
通过对烟草离体生长所需有机生长因子的系统研究。威斯康星第38号，Murashige和Skoog的修订培养基（RM-1962）通过删除吡哆醇、烟酸和L-甘氨酸而得到简化，并通过将硫胺素含量提高到400 /[mu]g/L而得到改进。生长素（吲哚乙酸）、激肽（激动素）和thaimine是必需的生长因子，肌醇是快速生长和高产所必需的。在这4种生长因子的适当水平下，获得约22 g/50 ml培养基的松散薄壁组织的鲜重产量，或获得较低产量的具有优异质量和活力的更致密组织。对于长寿来说，高泰敏水平尤其重要。与上述组合测试的其他因素给出以下结果：叶酸或对氨基苯甲酸显著提高产率;抗坏血酸和L-谷氨酸仅在与次优水平的硫胺素组合时增加产率;生物素和泛酸钙没有影响;核黄素是抑制性的。在RM-1964上，适当的生长素和激肽比例可使化学诱导的芽和根生长旺盛。在此培养基上，其它4个烟草品种的离体髓部组织和愈伤组织培养物均生长良好。在确定和指定组织培养的有机生长因子的要求的限制进行了讨论。
By a systematic study of organic growth factor requirements for in vitro growth of Nicotiana tabacum var. Wis. No. 38 the revised medium of Murashige and Skoog (RM-1962) has been simplified by deletion of pyridoxine, nicotinic acid and L-glycine and improved by raisingthe thiamine content to 400 /[mu]g/Liter. An auxin (indoleacetic acid), a kinin (kinetin), and thaimine were essential growth factors, and myo-inositol was required for rapid growth and high yields. With appropriate levels of these 4 growth factors, fresh weight yields of about 22 g/50 ml medium of loose parenchyma, or lower yields of more compact tissue of excellent quality and viability were obtained. For longevity a high thaimine level was especially important. Other factors tested in combinations with the above gave the following results folic acid or p-aminobenzoic acid significantly improved the yield; ascorbic acid and L-glutamic acid increased the yields only in combinations with suboptimal levels of thiamine; biotin and calcium-pantothenate were without effect; and riboflavin was inhibitory. With appropriate auxin kinin ratios, vigorous development of chemically-induced shoots and roots was obtained on RM-1964. Evidence is presented for satisfactory growth of excised pith tissue and callus cultures of 4 other tobacco varieties on this medium. Limitations in determining and specifying organic growth factor requirements for tissue cultures are discussed.