A Guide to Practical Histochemistry
A Guide to Practical Histochemistry
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实用组织化学指南
DOI：
10.1093/ajcp/55.3.389
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发表时间：
1971
影响因子：
3.5
通讯作者：
P. Melnick
中科院分区：
文献类型：
--
作者：
P. Melnick
这本261页的书中包含了大量的实际信息，显然已经在实验室中经过多年的应用测试。同时，包括简短，学术性和明确的原则描述，以便完成重要的目的，澄清组织化学中有意义的结果所需的重要步骤。书的前两部分包含关于固定的困难问题的相关材料，在章节中，要证明的化学成分和酶及其反应产物，以防止从细胞中扩散或浸出。在讨论的各种方法中，有快速冷冻的简单方法和使用氢键保护添加剂，如聚乙烯醇（PVA）。第二部分探讨了定量化的问题。下面简要介绍最常用的标准和特殊组织学染色。
The large amount of practical information compressed into this book of 261 pages has evidently been thoroughly tested by years of application in the laboratory. At the same time, brief, scholarly, and clear descriptions of principles are included, so that the important purpose is accomplished, of clarifying the significant steps required for meaningful results in histochemistry.The first two parts of the book contain pertinent material on the difficult problem of immobilizing, in the sections, the chemical components and enzymes to be demonstrated and their reaction products, to prevent diffusion or leaching from the cells. Among various approaches discussed are simple means for rapid freezing and the use of hydrogen-bonding protective additives such as polyvinyl alcohol (PVA). In the next part, problems of quantitation are touched upon. A brief section on the most frequently used standard and special stains of histology follows.