A NEURON-SPECIFIC ENHANCER OF THE DROSOPHILA DOPA DECARBOXYLASE GENE
A NEURON-SPECIFIC ENHANCER OF THE DROSOPHILA DOPA DECARBOXYLASE GENE
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DOI：
10.1101/gad.3.5.676
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发表时间：
1989-05-01
影响因子：
10.5
通讯作者：
HIRSH, J
中科院分区：
文献类型：
--
作者：
JOHNSON, WA;MCCORMICK, CA;HIRSH, J
至少有两个顺式调控元件是必要的正确的神经元特异性表达的果蝇多巴脱羧酶基因，DDC。除了先前描述的位于在mRNA起始位点上游60 bp处，我们现在已经表征了一个远端的600-pb的DNA片段，从-1019到-1623 bp，具有增强子样特性，是正常神经元特异性表达所必需的。表达缺失Ddc基因的神经元的免疫荧光标记表明，该区域包含一般的神经元调节元件和细胞特异性元件，选择性地影响Ddc在多巴胺能或多巴胺能神经元中的表达。这些选择性效应可以与去除由胚胎核提取物中存在的因子保护免于DNA酶消化的序列元件相关。这些元素中的几个也同源序列位于上游的进化分歧Ddc基因的果蝇virilis。这些结果表明，Ddc的神经元特异性表达的结果从结合在这个远端增强子区域内的几个因素的联合作用。
At least two cis-regulatory elements are necessary for correct neuron-specific expression of the Drosophila melanogaster dopa decaroxylase gene, Ddc. In addition to a previously described proximal element located .apprx. 60 bp upstream of the mRNA start site, we have now characterized a distal .apprx. 600-pb DNA fragment, extending from -1019 to -1623 bp, which possesses enhancer-like properties and is essential for normal neuron-specific expression. Immunofluorescent labeling of neurons expressing deleted Ddc genes indicates that this region contains both general neuronal regulatory elements and cell-specific elements that selectively affect Ddc expression in either dopaminergic or serotonergic neurons. These selective effects can be correlated with the removal of sequence elements that are protected from DNase digestion by factors present in embryonic nuclear extracts. Several of these elements are also homogolous to sequences located upstream of the evolutionarily diverged Ddc gene of Drosophila virilis. These results suggest that the neuron-specific expression of Ddc results from the combined action of several factors binding within this distal enhancer region.