Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR
Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR
复制标题
通过转移双子叶延伸因子 Tu 受体 EFR 增强单子叶水稻的先天免疫系统
DOI：
10.1111/jipb.12306
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发表时间：
2015-07-01
影响因子：
11.4
通讯作者：
Sun, Wenxian
中科院分区：
文献类型：
--
作者：
Lu, Fen;Wang, Huiqin;Sun, Wenxian
十字花科植物中的延伸因子Tu（EF-Tu）受体（EFR）特异性地识别细菌EF-Tu的N-末端乙酰化elf 18区域，从而激活植物免疫。已经证明拟南芥EFR在EFR转基因茄科植物中赋予广谱细菌抗性。在此，转基因水稻植物（Oryza sativa L. SSP. n.粳稻为了研究AtEFR基因是否能感知EF-Tu基因，从而提高单子叶植物的细菌抗性，构建了组成型表达AtEFR基因的单子叶植物细胞系。我们证明了来源于水稻黄单胞菌的elf 18肽分别在AtEFR转基因水稻细胞和植物中诱导氧化爆发和丝裂原活化蛋白激酶激活。在转基因水稻植株和细胞系中，致病相关基因如OsPBZ 1在elf 18刺激下显著上调。重要的是，用elf 18预处理引发了对X的强抗性。oryzaepv. AtEFR基因在水稻中的表达量与AtEFR基因的表达量密切相关。这些植株对水稻细菌性褐条病的抗性增强，但对稻瘟病的抗性没有增强。总的来说，结果表明具有AtEFR异源表达的水稻植物识别细菌EF-Tu并表现出增强的广谱细菌疾病抗性，并且模式识别受体介导的免疫可以在双子叶和单子叶两种植物中被操纵。
The elongation factor Tu (EF-Tu) receptor (EFR) in cruciferous plants specifically recognizes the N-terminal acetylated elf18 region of bacterial EF-Tu and thereby activates plant immunity. It has been demonstrated that Arabidopsis EFR confers broad-spectrum bacterial resistance in the EFR transgenic solanaceous plants. Here, the transgenic rice plants (Oryza sativa L. ssp. japonica cv. Zhonghua 17) and cell cultures with constitutive expression of AtEFR were developed to investigate whether AtEFR senses EF-Tu and thus enhances bacterial resistance in the monocot plants. We demonstrated that the Xanthomonas oryzae-derived elf18 peptide induced oxidative burst and mitogen-activated protein kinase activation in the AtEFR transgenic rice cells and plants, respectively. Pathogenesis-related genes, such as OsPBZ1, were upregulated dramatically in transgenic rice plant and cell lines in response to elf18 stimulation. Importantly, pretreatment with elf18 triggered strong resistance to X. oryzae pv. oryzae in the transgenic plants, which was largely dependent on the AtEFR expression level. These plants also exhibited enhanced resistance to rice bacterial brown stripe, but not to rice fungal blast. Collectively, the results indicate that the rice plants with heterologous expression of AtEFR recognize bacterial EF-Tu and exhibit enhanced broad-spectrum bacterial disease resistance and that pattern recognition receptor-mediated immunity may be manipulated across the two plant classes, dicots and monocots.