The Effects of Different Purifying Methods on the Chemical Properties, in Vitro Anti-Tumor and Immunomodulatory Activities of Abrus cantoniensis Polysaccharide Fractions.
The Effects of Different Purifying Methods on the Chemical Properties, in Vitro Anti-Tumor and Immunomodulatory Activities of Abrus cantoniensis Polysaccharide Fractions.
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DOI：
10.3390/ijms17040511
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发表时间：
2016-04-06
影响因子：
5.6
通讯作者：
Chun C
中科院分区：
文献类型：
--
作者：
Wu S;Fu X;Brennan MA;Brennan CS;Chun C
广东菜是一种受欢迎的中国蔬菜，可作为饮料、汤或民间药物食用。为了充分挖掘广东草多糖的潜力，对广东草多糖的9个部分（AP-AOH30-1、AP-AOH30-2、AP-AOH80-1、AP-AOH80-2、AP-ACl-1、AP-ACl-2、AP-ACl-3、AP-H和AP-L）进行了分离纯化。采用傅里叶变换红外光谱（FT-IR）和气相色谱（GC）对这些Abrus多糖组分（APF）进行了表征。采用秩和比（RSR）法对其体外抗肿瘤和免疫调节活性进行了研究和比较。结果表明，不同的APF在结构和生物活性方面存在显著差异，这与纯化工艺有关。APF中AP-ACl-3的产率为613.5 mg/kg，主要成分为鼠李糖（9.8%）、阿拉伯糖（8.9%）、果糖（3.0%）、半乳糖（9.9%）、葡萄糖（4.3%）、半乳糖醛酸（3.0%）和葡萄糖醛酸（61.1%），分子量为4.4 × 104 Da。此外，AP-ACl-3显示出相当大的生物活性，可显著阻止MCF-7细胞的迁移，刺激淋巴细胞增殖和腹腔巨噬细胞一氧化氮（NO）的产生。AP-ACl-3是一种潜在的新型抗肿瘤和免疫调节剂。
Abrus cantoniensis (Hance) is a popular Chinese vegetable consumed as a beverage, soup or folk medicine. To fully exploit the potential of the polysaccharide in Abrus cantoniensis, nine polysaccharide fractions of Abrus cantoniensis were isolated and purified (AP-AOH30-1, AP-AOH30-2, AP-AOH80-1, AP-AOH80-2, AP-ACl-1, AP-ACl-2, AP-ACl-3, AP-H and AP-L). Fourier-transform infrared spectroscopy (FT-IR) and gas chromatography (GC) were used to characterize these Abrus polysaccharides fractions (APF). In vitro anti-tumor and immunomodulatory activities were also investigated and compared using the rank-sum ratio (RSR) method. Results demonstrated significant differences in the structure and bioactivities among APF, which were associated to the process used for their purification. Among the APF, AP-ACl-3 yield was 613.5 mg/kg of product and consisted of rhamnose (9.8%), arabinose (8.9%), fructose (3.0%), galactose (9.9%), glucose (4.3%), galacturonic acid (3.0%) and glucuronic acid (61.1%) with a molecular weight of 4.4 × 104 Da. Furthermore, AP-ACl-3 exhibited considerable bioactivities significantly preventing the migration of MCF-7 cells and stimulating lymphocyte proliferation along with nitric oxide (NO) production of peritoneal macrophages. AP-ACl-3 could be explored as a novel potential anti-tumor and immunomodulatory agent.