Phenotype of thymic stromal cells. An immunoelectron microscopic study with anti-IA, anti-MAC-1, and anti-MAC-2 antibodies.
Phenotype of thymic stromal cells. An immunoelectron microscopic study with anti-IA, anti-MAC-1, and anti-MAC-2 antibodies.
复制标题
胸腺基质细胞的表型。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
M. Papiernik
中科院分区：
文献类型：
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作者：
B. Nabarra;M. Papiernik
为了更好地理解胸腺微环境，有必要确定参与胸腺内T细胞分化的形成胸腺网的细胞的抗原谱。这些细胞有三种类型：上皮细胞、巨噬细胞和交错突细胞（IDC）。虽然已经在光镜下对胸腺切片进行了几项研究，但尚未明确分析阳性细胞的鉴定，主要是巨噬细胞和IDC的抗原设备。形态学，在电子显微镜是迄今为止最好的方法，以确定不同类型的细胞，和胸腺切片上的免疫电镜可能是最好的方法，以明确定义基质细胞表型。在本文中，我们分析了经典定义的巨噬细胞家族，Mac-1和Mac-2，以及主要组织相容性复合物II类抗原，这是目前的上皮细胞和骨髓来源的基质细胞上的两个抗原。结果表明，上皮细胞为Ia+ Mac-1-、Mac-2-;巨噬细胞均为Mac-1+、Mac-2+，但只有一半为Ia+; IDC为Ia+、Mac-1+、Mac-2+。这些结果表明IDC和巨噬细胞都表达最初被描述为巨噬细胞特异性的抗原。
To better comprehend the thymic microenvironment, it is necessary to identify the antigenic profile of cells forming the thymic reticulum which are involved in intrathymic T cell differentiation. These cells are of three types: epithelial cells, macrophages, and interdigitating cells (IDC). Although several studies have been done on thymus section in light microscopy, identification of the positive cells, and mainly the antigenic equipment of the macrophages and IDC has not been clearly analyzed. Morphology, in electron microscopy is so far the best method to identify the different types of cells, and immunoelectron microscopy on thymic sections may be the best method to define clearly stromal cell phenotypes. In the present paper, we analyzed two antigens which classically define the macrophage family, Mac-1 and Mac-2, as well as major histocompatibility complex class II antigen which is present on epithelial cells and on bone marrow derived stromal cells. We show that epithelial cells are Ia+ Mac-1-, Mac-2-; macrophages are all Mac-1+, Mac-2+ but only half are Ia+; IDC are Ia+, Mac-1+, Mac-2+. These results show that IDC and macrophages both express antigens which were originally described as macrophage-specific.