Transgene-based, female-specific lethality system for genetic sexing of the silkworm, Bombyx mori
Transgene-based, female-specific lethality system for genetic sexing of the silkworm, Bombyx mori
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基于转基因的雌性特异性致死系统，用于家蚕 Bombyx mori 的遗传性别鉴定
DOI：
10.1073/pnas.1221700110
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发表时间：
2013-04-23
影响因子：
11.1
通讯作者：
Huang, Yongping
中科院分区：
文献类型：
--
作者：
Tan, Anjiang;Fu, Guoliang;Huang, Yongping
正在为具有农业和公共卫生重要性的昆虫开发基于转基因的遗传性别鉴定方法。长期以来，在蚕业中一直寻求只饲养雄性，因为雄性具有更好的健康状况、更低的食物消耗和更高的丝产量等优越的经济特征。在这里，我们报告了一个基于转基因的家蚕遗传性别鉴定系统的建立。我们开发了一种结构，在这种结构中，由性别特异性选择性剪接调节的正反馈环导致四环素可抑制反式激活剂仅在女性中高水平表达。转基因动物在胚胎和早期幼虫阶段表现出雌性特有的致死性，导致只有雄性蚕茧。这种基于转基因的雌虫特异性致死系统不仅在蚕业上有广泛的应用，而且在鳞翅目害虫防治方面也有很大的潜力。
Transgene-based genetic sexing methods are being developed for insects of agricultural and public health importance. Male-only rearing has long been sought in sericulture because males show superior economic characteristics, such as better fitness, lower food consumption, and higher silk yield. Here we report the establishment of a transgene-based genetic sexing system for the silkworm, Bombyx mori. We developed a construct in which a positive feedback loop regulated by sex-specific alternative splicing leads to high-level expression of the tetracycline-repressible transactivator in females only. Transgenic animals show female-specific lethality during embryonic and early larval stages, leading to male-only cocoons. This transgene-based female-specific lethal system not only has wide application in sericulture, but also has great potential in lepidopteran pest control.