Quantitative analysis of human telomerase reverse transcriptase in pancreatic cancer
Quantitative analysis of human telomerase reverse transcriptase in pancreatic cancer
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DOI：
10.1158/1078-0432.ccr-05-1821
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发表时间：
2006-04-01
影响因子：
11.5
通讯作者：
Tanaka, M
中科院分区：
文献类型：
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作者：
Ohuchida, K;Mizumoto, K;Tanaka, M
虽然端粒酶活性是一个很有前景的诊断标志物，但由于缺乏评估样本质量的手段，临床上将这一标志物用于癌症诊断仍然存在问题。人端粒酶逆转录酶（hTERT）是端粒酶的亚基之一，也是一种很有前景的诊断标志物。在本研究中，我们对88份胰腺液样本进行了大规模分析，以确定定量分析hTERT mRNA诊断胰腺癌的可行性。我们发现hTERT在癌源性、导管内乳头状粘液瘤（IPMN）源性和慢性胰腺炎源性果汁样本中的表达有显著差异。结果表明，定量分析hTERT mrna在鉴别胰腺癌和IPMN方面比鉴别慢性胰腺炎更有用。当特异性设置为100%时，癌与IPMN鉴别的敏感性为43.5%，而细胞学检查的敏感性为22.0%。显微解剖分离的胰腺组织癌细胞、IPMN细胞和正常导管细胞中hTERT的表达有显著差异。从胰腺炎组织学表现的组织中分离的淋巴细胞和增生性上皮细胞显示不同水平的hTERT表达。我们的结果表明，定量分析胰液中hTERT mRNA比细胞学分析更有利于区分癌和IPMN，但可能不利于区分癌和慢性胰腺炎。
Although telomerase activity is a promising diagnostic marker, clinical introduction of this marker for cancer diagnosis is still problematic due to the lack of means of evaluating sample quality. Human telomerase reverse transcriptase (hTERT), one of the subunits of telomerase, is also a promising diagnostic marker. In the present study, we did large-scale analysis of 88 pancreatic juice samples to determine the feasibility of quantitative analysis of hTERT mRNA for diagnosis of pancreatic cancer. We found significant differences in hTERT expression among carcinoma-derived, intraductal papillary mucinous neoplasm (IPMN)-derived, and chronic pancreatitis-derived juice samples. Results showed that quantitative analyses of hTERT mRNAs are more useful in discriminating carcinoma from IPMN than from chronic pancreatitis. When the specificity was set at 100%, the sensitivity for differentiation between carcinoma and IPMN was 43.5%, whereas the sensitivity of cytologic examination was 22.0%. There were significant differences in hTERT expression among carcinoma cells, IPMN cells, and normal ductal cells isolated from pancreatic tissues by microdissection. Lymphocytes and hyperplastic epithelial cells isolated from tissues with the histologic appearance of pancreatitis showed various expression levels of hTERT Our results suggest that quantitative analysis of hTERT mRNA in pancreatic juice is advantageous over cytologic analysis for differentiation between carcinoma and IPMN but probably not for differentiation between carcinoma and chronic pancreatitis.