Induction of apoptosis by canine natural killer cells.
Induction of apoptosis by canine natural killer cells.
复制标题
犬自然杀伤细胞诱导细胞凋亡。
DOI：
10.1046/j.1439-0442.2003.00517.x
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
E. Burkhardt
中科院分区：
文献类型：
--
作者：
G. Schmitz;A. Armién;S. Fonfara;J. Teifke;E. Burkhardt
Besides a secretory pathway of canine natural killer (NK) cells, which results in necrosis of the target cell, a second pathway was demonstrated, which results in apoptosis of the target cell. Comparing the Chromium Release Assay (CRA) and the Rose Bengal Assay (RBA) for quantification of in vitro canine NK cell activity, a constant 10% higher NK cell activity was found in the RBA compared with the CRA. To find out the mechanism responsible for the different results of both tests, morphological studies of in vitro canine NK cell activity against epithelial and mesenchymal allogenic target cell lines were performed. Most target cells were undergoing necrosis as a result of NK cell killing, which was evidenced by transmission electron microscopy. However, besides necrotic target cells, shrunken target cells with dense cytoplasm, fragmented nuclei and disruption into membrane-bound bodies were detected, which are known as signs of apoptosis. Additionally, using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) method, 13-23% of target cells presented a positive staining, indicative of apoptosis. These findings give evidence for the ability of canine NK cells to kill their target cells via two different pathways, which results either in apoptosis or necrosis.
人类 NK 细胞的非分泌性凋亡杀伤。
DOI：
--
发表时间：
1996
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Vujanovic,NL;Nagashima,S;Herberman,RB;Whiteside,TL
通讯作者：
Whiteside,TL
吸入亚硝酸异丁酯通过 T 细胞独立机制损害 T 细胞功能。
影响因子：
3.5
作者：
Soderberg,LS
通讯作者：
Soderberg,LS