Prophage induction of indigenous marine lysogenic bacteria by environmental pollutants
Prophage induction of indigenous marine lysogenic bacteria by environmental pollutants
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DOI：
10.3354/meps164125
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发表时间：
1998-01-01
影响因子：
2.5
通讯作者：
Paul, JH
中科院分区：
文献类型：
--
作者：
Cochran, PK;Kellogg, CA;Paul, JH
溶源细菌可能是海洋沃茨细菌群落中丰富的组成部分。在河口和其他富营养化环境中发现的大量病毒可能是部分诱导原噬菌体的结果。丝裂霉素C是选择用于原噬菌体诱导的诱导剂;然而，这在海洋环境中不是天然存在的。我们确定了对环境重要的污染物影响海洋细菌自然种群中原噬菌体诱导的能力。我们调查了多氯联苯1248，一种多氯联苯混合物，掩体C燃料油#6，和农药混合物作为诱导剂的天然细菌群落从墨西哥湾。丝裂霉素C也用作诱导的阳性对照。与对照组相比，诱导组的病毒直接计数显著增加，范围为对照组的149 - 1336%。三分之二的环境采样显示原噬菌体诱导所使用的方法之一，与PCB混合物和Aroclor 1248给予最高的百分比效率（75%）的诱导。这项研究表明，许多环境重要的污染物可能是诱导剂自然溶原性病毒生产的海洋环境。
Lysogenic bacteria may be abundant components of bacterial assemblages in marine waters. The tremendous number of viruses found in estuarine and other eutrophic environments may be the result in part of induction of prophages. Mitomycin C is the inducing agent of choice for prophage induction; however this is not naturally found in the marine environment. We determined the capability of environmentally important pollutants to effect prophage induction in natural populations of marine bacteria. We investigated Aroclor 1248, a PCB mixture, bunker C fuel oil #6, and a pesticide mixture as inducing agents for natural bacterial communities from the Gulf of Mexico. Mitomycin C was also employed as a positive control for induction. Induction was determined as a significant increase in viral direct counts compared to control and ranged from 149 to 1336% of the controls. Two-thirds of the environments sampled showed prophage induction by one of the methods utilized, with the PCB mixture and Aroclor 1248 giving the highest percent efficiency (75%) of induction. This study shows that many environmentally important pollutants may be inducing agents for natural lysogenic viral production in the marine environment.