Isolation and characterization of a C-type lectin cDNA specifically expressed in the tip of mouthparts of the flesh fly Sarcophaga peregrina
Isolation and characterization of a C-type lectin cDNA specifically expressed in the tip of mouthparts of the flesh fly Sarcophaga peregrina
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DOI：
10.1111/j.0962-1075.2004.00468.x
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发表时间：
2004-04-01
影响因子：
2.6
通讯作者：
Kotani, E
中科院分区：
文献类型：
--
作者：
Yamamoto-Kihara, M;Kotani, E
我们已经分离出一个新的基因，CLEM 36，肉蝇Sarcophaga peregrina，这表明显着的同源性的C型凝集素家族。从羽化后第二天开始，CLEM 36 mRNA仅在口器顶端转录过量。整体原位杂交结果显示CLEM 36 mRNA在位于食物道入口处和唇瓣与Haustellum之间的C型凝集素产生组织（CLPT）中表达。免疫印迹分析表明，CLEM 36蛋白的成熟形式在CLPT中合成，然后分泌到唾液中。我们的研究结果表明，CLEM 36蛋白可能在该昆虫对病原体的生物防御中发挥重要作用。
We have isolated a novel gene, CLEM 36, of the flesh fly Sarcophaga peregrina, which shows significant homology to the C-type lectin family. CLEM 36 mRNA was transcribed excessively from the second day after eclosion only in the tip of mouthparts. Whole mount in situ hybridization showed that CLEM 36 mRNA was expressed in the C-type lectin-producing tissue (CLPT) located at the entrance of the food canal and between the labellum and haustellum. Immunoblot analysis showed that the mature form of CLEM 36 protein was synthesized in the CLPT, then secreted into saliva. Our results indicate that CLEM 36 protein may play an important role in biological defence against pathogens during the food intake of this insect.