Proteins synthesized and secreted by larvae of the ectoparasitic wasp, Eulophus pennicornis.
Proteins synthesized and secreted by larvae of the ectoparasitic wasp, Eulophus pennicornis.
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DOI：
10.1002/arch.1024
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发表时间：
2001-03
影响因子：
2.2
通讯作者：
E. H. Richards;J. P. Edwards
中科院分区：
文献类型：
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作者：
E. H. Richards;J. P. Edwards
通过对寄主甘蓝小卷蛾幼虫的显微镜观察，发现蠕动波从取食小卷蛾幼虫的前端向后端传播，反之亦然。此外，当黄蜂幼虫在体外浸泡在PBS中时，它们释放出各种蛋白质，分子量范围为（至少）14至200 kDa。其中有一种蛋白质的分子量估计与寄生L. [理查兹和爱德华兹，昆虫生物化学分子生物学29：557-569（1999）]。当黄蜂幼虫在浸泡在组织培养基或蔗糖中的棉絮球上孵育时，也得到了类似的结果，类似于它们的自然进食行为。这些结果（以及其他结果）表明，黄蜂幼虫通过口腔释放蛋白质。利用（35）S-蛋氨酸进行的蛋白质合成研究表明，该蜂幼虫在体外合成并分泌多种蛋白质，包括一种分子量与L.甘蓝27 kDa PSP。正如预期的那样，只有一部分的总蛋白质合成的寄生虫幼虫随后分泌。此外，分泌的蛋白质的放射自显影包含显着较少的带比银染色的SDS凝胶的蛋白质释放到PBS或棉絮。因此，在后一种凝胶上检测到的一些额外条带被认为代表不是黄蜂来源的蛋白质。相反，这些由黄蜂幼虫释放的蛋白质被推测是来自它们的肠道，因此，可能代表来自宿主血淋巴和进食过程中摄入的蛋白质。这种可能性得到了支持的匀浆上清液的电泳分析，从黄蜂幼虫与或不与他们的肠道内容物。这些研究表明，肠道内容物的幼虫寄生蜂贡献了几个不同的条带的总蛋白质谱。E.并结合内寄生蜂幼虫的蛋白质合成、分泌和释放过程进行了讨论。出版于2001年Wiley-Liss，Inc.
A microscopic examination of Eulophus pennicornis larvae on their host Lacanobia oleracea, revealed that peristaltic waves travelled from the anterior to posterior end of the feeding wasp larvae, and vice versa. In addition, when wasp larvae were immersed in PBS in vitro, they released a variety of proteins, with molecular weights ranging from (at least) 14 to 200 kDa. Amongst these was a protein with an estimated molecular weight similar to that of the 27 kDa parasitism-specific protein (PSP) detected in plasma from parasitized L. oleracea [Richards and Edwards, Insect Biochem Mol Biol 29:557-569 (1999)]. Similar results were obtained when the wasp larvae were incubated on balls of cotton wool soaked in tissue culture medium or sucrose, i.e., conditions that resemble their natural feeding behaviour. These results (and others) indicate that the wasp larvae release proteins, putatively through their mouth. Protein synthesis studies using (35)S-methionine indicated that the wasp larvae synthesize and secrete a variety of proteins in vitro, including one with a molecular weight corresponding to that of the L. oleracea 27 kDa PSP. As expected, only a portion of the total proteins synthesized by the parasitoid larvae were subsequently secreted. In addition, the autoradiogram of secreted proteins contained significantly fewer bands than silver-stained SDS gels of proteins released into PBS or onto cotton wool. Thus, some of the additional bands detected on the latter gels are thought to represent proteins that were not of wasp origin. Instead, these proteins released by the wasp larvae are speculated to be derived from their gut and, as such, probably represent proteins derived from host haemolymph and ingested during feeding. This possibility was supported by an electrophoretic analysis of homogenate supernatants prepared from wasp larvae with or without their gut contents. These studies indicated that the gut contents of the larval parasitoid contributes several distinct bands to the total protein profile. The ability of E. pennicornis larvae to synthesize, secrete, and release proteins is discussed with reference to those produced by endoparasitoid larvae. Published 2001 Wiley-Liss, Inc.