Effects of Passive Stretching on Muscle Injury and HSP Expression during Recovery after Immobilization in Rats
Effects of Passive Stretching on Muscle Injury and HSP Expression during Recovery after Immobilization in Rats
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DOI：
10.1159/000228901
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发表时间：
2009-10
期刊：
影响因子：
5
通讯作者：
Takayuki Inoue;Shigeyuki Suzuki;Ryusuke Hagiwara;M. Iwata;Yasuhiro Banno;M. Okita
中科院分区：
文献类型：
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作者：
Takayuki Inoue;Shigeyuki Suzuki;Ryusuke Hagiwara;M. Iwata;Yasuhiro Banno;M. Okita
目的：众所周知，伸展运动会引起肌肉肥大，并与肌纤维行为的调节有关。我们的目的是确定伸展运动是否可以保护固定的大鼠比目鱼肌免受重新加载引起的肌肉损伤。方法：将 54 只 8 周龄雄性 Wistar 大鼠的后肢用石膏固定 4 周，然后对 24 只大鼠（NS 组）通过正常行走单独重新加载，对 25 只大鼠（S 组）进行被动拉伸后进行被动拉伸。伸展运动（每天30分钟）持续6天。为了确定在石膏固定后重新加载过程中被动拉伸是否会影响大鼠比目鱼肌中热休克蛋白（HSP）的表达，在石膏去除后测量了侵入肌纤维和热休克蛋白表达的比率。结果：NS组在重新加载的第一天和第二天，侵入肌纤维的比例增加。与单独重新加载相比，在石膏去除后的大多数时间点，伸展运动减少了侵入的肌纤维（S组）。此外，仅在没有被动拉伸的组（NS组）中，HSP25和HSP72的表达在重新加载期间随时间增加。结论：固定后，大鼠比目鱼肌被动伸展运动可防止重新加载引起的损伤。此外，被动拉伸提供的保护独立于 HSP。
Objective: Stretching exercise is known to induce muscle hypertrophy and is implicated in the modulation of muscle fiber behavior. We aim to determine whether stretching exercise is protective against reloading-induced muscle damage in immobilized rat soleus muscle. Methods: Rat hindlimbs in 54 eight-week-old male Wistar rats were immobilized by cast for 4 weeks, followed by reloading alone through normal ambulation in 24 (group NS) and after passive stretch in 25 rats (group S). Stretching exercise (30 min each day) lasted 6 days. To determine if passive stretching affects expression of heat shock proteins (HSPs) in rat soleus muscle during reloading following cast immobilization, the ratio of invading muscle fibers and HSPs expression were measured following cast removal. Results: The ratio of invading muscle fibers increased during the first and second days of reloading in group NS. Compared with reloading alone, stretching exercise reduced invading muscle fibers at most time points following cast removal (group S). Additionally, expression of HSP25 and HSP72 increased with time during reloading only in the group without passive stretch (group NS). Conclusion: Following immobilization, in the rat soleus muscle passive stretching exercise protects against injury induced by reloading. Furthermore, the protection provided by passive stretch is independent of HSPs.