Label-Free Aptamer-Based Immunoglobulin Sensors Using Graphene Field-Effect Transistors
Label-Free Aptamer-Based Immunoglobulin Sensors Using Graphene Field-Effect Transistors
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DOI：
10.1143/jjap.50.070120
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发表时间：
2011-07-01
影响因子：
1.5
通讯作者：
Matsumoto, Kazuhiko
中科院分区：
文献类型：
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作者：
Ohno, Yasuhide;Maehashi, Kenzo;Matsumoto, Kazuhiko
使用适配体修饰的石墨烯场效应晶体管（g - fet）演示了特定蛋白质的电检测。免疫球蛋白E （IgE）适配体以1-芘丁酸琥珀酰亚胺酯为连接剂固定在石墨烯表面。从原子力显微镜图像中，石墨烯通道的高度被确定为大约3纳米，表明适配体的成功功能化。适配体功能化前后输运特性的斜率没有变化，说明功能化过程没有引入缺陷。适配体修饰的G-FET只检测到目标蛋白，而各种蛋白改变了裸G-FET的漏极电流。这些结果表明，非靶蛋白与石墨烯通道表面的结合被充分抑制。(C) 2011 .日本应用物理学会
Electrical detection of specific proteins was demonstrated using aptamer-modified graphene field-effect transistors (G-FETs). Immunoglobulin E (IgE) aptamers were immobilized onto the graphene surface with 1-pyrenebutanoic acid succinimidyl ester as a linker. From an atomic-force microscopy image, the height of the graphene channel was determined to be approximately 3 nm, indicating the successful functionalization of aptamers. The slope of the transport characteristics before and after aptamer functionalization did not change, indicating that the functionalization process was carried out without introducing defects. The aptamer-modified G-FET successfully detected only the target protein while the drain current of the bare G-FETs changed by various proteins. These results suggest that the binding of the non-target protein to the graphene channel surface was sufficiently suppressed. (C) 2011 The Japan Society of Applied Physics