Podoplanin expression in primary central nervous system germ cell tumors: a useful histological marker for the diagnosis of germinoma
Podoplanin expression in primary central nervous system germ cell tumors: a useful histological marker for the diagnosis of germinoma
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DOI：
10.1007/s00401-006-0033-4
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发表时间：
2006-06-01
影响因子：
12.7
通讯作者：
Matsutani, Masao
中科院分区：
文献类型：
--
作者：
Mishima, Kazuhiko;Kato, Yukinari;Matsutani, Masao
Podoplanin是一种粘蛋白样跨膜唾液酸糖蛋白，可促进血小板聚集，并可能参与癌细胞的迁移、侵袭、转移和恶性进展。Podoplanin/aggrus在睾丸腺瘤中高表达，提示其可能是睾丸腺瘤的敏感标志物。本文采用免疫组化方法检测了62例中枢神经系统生殖细胞肿瘤中podoplanin的表达。在40/41例（98%）生殖细胞瘤（包括混合GCT中的生殖细胞瘤成分）中，podoplanin在生殖细胞瘤细胞表面弥漫表达;淋巴细胞、间质细胞和合体滋养层巨细胞对podoplanin呈阴性。除未成熟畸胎瘤（12/17; 71%）外，podoplanin在非生殖细胞瘤GCT中不表达，包括7例畸胎瘤，7例胚胎癌，7例卵黄囊瘤和7例绒毛膜癌。在未成熟畸胎瘤中，在不到10%的未成熟鳞状和柱状上皮细胞中观察到局灶性podoplanin染色。因此，podoplanin表达可能是CNS GCT中生殖细胞瘤的敏感免疫组化标记物。因此，它可用于诊断，用于监测治疗的功效，并作为基于抗体的治疗的潜在靶标。
Podoplanin, a mucin-like transmembrane sialoglycoprotein, promotes platelet aggregation and may be involved in cancer cell migration, invasion, metastasis, and malignant progression. Podoplanin/aggrus is highly expressed in testicular seminoma, suggesting that it may be a sensitive marker for testicular seminomas. Here we investigated the expression of podoplanin in central nervous system (CNS) germ cell tumors (GCTs) by immunohistochemical staining of tumor samples from 62 patients. In 40 of 41 (98%) germinomas (including germinomatous components in mixed GCTs), podoplanin was diffusely expressed on the surface of germinoma cells; lymphocytes, interstitial cells, and syncytiotrophoblastic giant cells were negative for podoplanin. Except for immature teratomas (12/17; 71%), podoplanin expression was absent in non-germinomatous GCTs, including seven teratomas, seven embryonal carcinomas, seven yolk sac tumors, and seven choriocarcinomas. In immature teratomas, focal podoplanin staining was observed in fewer than 10% of immature squamous and columnar epithelial cells. Thus, podoplanin expression may be a sensitive immunohistochemical marker for germinoma in CNS GCTs. As such, it may be useful for diagnosis, for monitoring the efficacy of treatment, and as a potential target for antibody-based therapy.