Differential responses of gut microbiota to the same prebiotic formula in oligotrophic and eutrophic batch fermentation systems.
Differential responses of gut microbiota to the same prebiotic formula in oligotrophic and eutrophic batch fermentation systems.
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寡营养和富营养分批发酵系统中肠道微生物群对相同益生元配方的不同反应
DOI：
10.1038/srep13469
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发表时间：
2015-08-25
影响因子：
4.6
通讯作者：
Pang X
中科院分区：
文献类型：
--
作者：
Long W;Xue Z;Zhang Q;Feng Z;Bridgewater L;Wang L;Zhao L;Pang X
当在不同批次发酵研究中进行评估时，相同的益生元对微生物群产生不一致的影响。为了理解这些差异背后的原因，我们比较了一种益生元配方在两种常用的分批系统中对相同接种的粪便微生物群的影响：磷酸盐缓冲盐水（PBS，贫营养）和基础培养基（BHP，富营养）。使用454焦磷酸测序监测微生物群。两种系统的阴性对照（无益生元）在发酵过程中均显示出微生物群的显著变化，尽管它们的pH值保持相对稳定，尤其是在大肠杆菌中，嗜胆汁菌和埃希氏菌/志贺氏菌增加，但粪杆菌减少。我们通过包括基线和阴性对照的冗余分析来鉴定益生元应答者。两个系统中的关键阳性和阴性应答者非常不同，只有8个一致调节的OTU（28个阳性应答者中的7个和35个阴性应答者中的1个）。此外，同一属内的一些OTU以相反的方式对益生元作出反应。因此，为了获得益生元对微生物群的调节作用的完整的体外评价，有必要使用贫营养和富营养系统，将处理组与基线和阴性对照进行比较，并分析微生物群变化至OTU水平。
The same prebiotics have produced inconsistent effects on microbiota when evaluated in different batch fermentation studies. To understand the reasons behind these discrepancies, we compared impact of one prebiotic formula on the same inoculated fecal microbiota in two frequently used batch systems: phosphate-buffered saline (PBS, oligotrophic) and basal culture medium (BCM, eutrophic). The microbiota was monitored using 454 pyrosequencing. Negative controls (no prebiotic) of both systems showed significant shifts in the microbiota during fermentation, although their pH remained relatively stable, especially in BCM, with increases inBilophilaandEscherichia/Shigellabut a decrease inFaecalibacterium. We identified prebiotic responders via redundancy analysis by including both baseline and negative controls. The key positive and negative responders in the two systems were very different, with only 8 consistently modulated OTUs (7 of the 28 positive responders and 1 of the 35 negative responders). Moreover, some OTUs within the same genus responded to the prebiotic in opposite ways. Therefore, to obtain a completein vitroevaluation of the modulatory effects of a prebiotic on microbiota, it is necessary to use both oligotrophic and eutrophic systems, compare treatment groups with both baseline and negative controls and analyze the microbiota changes down to the OTU level.