Modified cellular immune responses in dogs infected with Echinococcus multilocularis
Modified cellular immune responses in dogs infected with Echinococcus multilocularis
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DOI：
10.1007/s00436-005-1303-0
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发表时间：
2005-03-01
影响因子：
2
通讯作者：
Kamiya, M
中科院分区：
文献类型：
--
作者：
Kato, N;Nonaka, N;Kamiya, M
研究了经口接种泡球蚴的犬的寄生虫特异性抗原应答和淋巴细胞转化。从感染后7天（DPI），血清IgG 1（Th 2-导向）和IgG 2（Th 1-导向）水平对体细胞和排泄分泌（ES）抗原的原头节和蠕虫增加，与最高的反应对原头节排泄分泌抗原（PES）。在21天的感染期间，未观察到外周血单核细胞（PBMC）对寄生虫抗原的特异性胚细胞生成，但在21 DPI时，两只犬中有一只的派伊尔集合淋巴结细胞显示出对PES的胚细胞生成（刺激指数：4.65）。有趣的是，仅在7 DPI时伴刀豆球蛋白A（ConA）诱导的PBMC增殖反应降低。此外，ConA诱导的增殖反应的淋巴细胞从各种来源被抑制的寄生虫抗原，特别是PES。这些数据表明，虽然在E.多房绦虫感染犬，寄生虫抗原，特别是PES，可能对这些犬的淋巴细胞反应具有不完全抑制作用。
Parasite-specific antigen responses and lymphocyte blastogenesis in dogs orally inoculated with Echinococcus multilocuralis metacestodes were examined. Serum IgG1 (Th2-oriented) and IgG2 (Th 1-oriented) levels against somatic and excretory-secretory (ES) antigens of protoscoleces and adult worms increased from 7 days post-infection (DPI), with the highest responses against protoscolex excretory-secretory antigen (PES). Specific blastogenesis of peripheral blood mononuclear cells (PBMC) against the parasite antigens was not observed during the 21-day infection period, but Peyer's patches cells from one out of two dogs at 21 DPI showed blastogenesis against PES (stimulation index: 4.65). Interestingly, only at 7 DPI were concanavalin A (ConA)-induce proliferative responses of PBMC reduced. Moreover, ConA-induced proliferative responses of lymphocytes from various origins were suppressed by the addition of parasite antigens, especially with PES. These data suggest that although both Th1- and Th2-oriented humoral immune responses were observed in E. multilocularis infected dogs, the parasite antigens, especially PES, may have incompletely suppressed lymphocyte responses in these dogs.