Functional roles of Notch signaling in the cnidarian Nematostella vectensis
Functional roles of Notch signaling in the cnidarian Nematostella vectensis
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DOI：
10.1016/j.ydbio.2011.11.012
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发表时间：
2012-02-15
影响因子：
2.7
通讯作者：
Martindale, Mark Q.
中科院分区：
文献类型：
--
作者：
Marlow, Heather;Roettinger, Eric;Martindale, Mark Q.
Notch 信号传导是已知最古老的后生动物信号传导途径之一，在多种发育环境中使用，以影响细胞分化、规范和干细胞状态的维持。在这里，我们报告了早期分支后生动物 Nematostella vectensis（Anthozoa、Cnidaria）在胚胎和幼虫发育过程中经典 Notch 信号通路的分离和表达。我们使用药物治疗、吗啉代敲低和显性失活错误表达实验来证明Notch信号传导可以介导刺胞动物发生，即刺胞动物特异性神经效应细胞的发育。 Notch 信号传导通常会导致 NvHes 基因（bHLH 转录因子的保守家族）的转录激活。通过药理抑制或敲低 Notch 效应基因 Suppressor of Hairless Su(H) 而导致 Notch 信号传导丧失，同样会导致刺胞细胞命运的丧失。我们还提供证据表明，Notch 信号传导对发育中的 N. vectensis planula 的神经发生的某些方面负责，其中通过药理学试剂 DAPT 破坏 Notch 裂解导致体内神经标记基因表达增加。该数据表明，Notch 信号传导作用于发育中的神经系统的组成部分，是该通路的一个古老的角色。 Notch 信号对于刺胞细胞和神经元发育的共同需求进一步支持了刺胞细胞和神经元作为多功能感觉效应细胞具有共同起源的假设。 (C) 2011 Elsevier Inc. 保留所有权利。
Notch signaling is among the oldest of known Metazoan signaling pathways and is used in a multitude of developmental contexts to effect cellular differentiation, specification and the maintenance of stem cell state. Here we report the isolation and expression of the canonical Notch signaling pathway in the early branching metazoan Nematostella vectensis (Anthozoa, Cnidaria) during embryonic and larval development. We have used pharmacological treatment, morpholino knockdown, and dominant negative misexpression experiments to demonstrate that Notch signaling acts to mediate cnidogenesis, the development of cnidarian-specific neural effecter cells. Notch signaling often results in the transcriptional activation of NvHes genes, a conserved family of bHLH transcription factors. A loss of Notch signaling through use of pharmacological inhibition or knock-down of the Notch effecter gene Suppressor of Hairless Su(H) similarly results in a loss of cnidocyte cell fate. We also provide evidence that Notch signaling is responsible for certain aspects of neurogenesis in developing N. vectensis planula in which disruption of Notch cleavage via the pharmacological agent DAPT results in increased expression of neural marker genes in vivo. This data suggests that Notch signaling acting on components of the developing nervous system is an ancient role of this pathway. The shared requirement of Notch signaling for the development of both cnidocytes and neurons further supports the hypothesis that cnidocytes and neurons share common origins as multifunctional sensory-effecter cells. (C) 2011 Elsevier Inc. All rights reserved.