Altered basic fibroblast growth factor expression in common epidermal neoplasms: examination with in situ hybridization and immunohistochemistry.
Altered basic fibroblast growth factor expression in common epidermal neoplasms: examination with in situ hybridization and immunohistochemistry.
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DOI：
10.1067/mjd.2000.104792
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发表时间：
2000-06
影响因子：
13.8
通讯作者：
J. Arbiser;H. Byers;C. Cohen;J. Arbeit
中科院分区：
文献类型：
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作者：
J. Arbiser;H. Byers;C. Cohen;J. Arbeit
背景成纤维细胞生长因子家族包括酸性成纤维细胞生长因子、碱性成纤维细胞生长因子和卡波西成纤维细胞生长因子。这些生长因子在皮肤病中的分布尚未确定。目的探讨这些生长因子在角质形成细胞皮损和正常皮肤中的分布。方法对常见角质形成细胞疾病的皮肤切片，采用aFGFbFGFkFGF3种特异性探针进行原位杂交和免疫组织化学检测。结果在所研究的生长因子中，只有bFGF存在于皮肤中。碱性成纤维细胞生长因子信使RNA在正常表皮和良、恶性上皮性肿瘤中均有高表达。在正常皮肤中，碱性成纤维细胞生长因子主要以基底以上的方式表达，而在上皮性肿瘤中，则观察到均匀的高水平表达。结论碱性成纤维细胞生长因子是皮肤表达的成纤维细胞生长因子的主要成员。碱性成纤维细胞生长因子的合成来源是角质形成细胞。在正常皮肤中，碱性成纤维细胞生长因子的免疫反应主要局限于表皮的上层，但在良性肿瘤和恶性肿瘤的情况下，碱性成纤维细胞生长因子在表皮的所有层都有表达。促进上皮性肿瘤的基因变化也可能促进bFGF信使RNA向蛋白质的转化。特异性抑制碱性成纤维细胞生长因子的活性可能在常见皮肤病的治疗中应用。
BACKGROUND The fibroblast growth factor family consists of acidic fibroblast growth factor (aFGF), basic fibroblast growth factor (bFGF), and Kaposi fibroblast growth factor (kFGF). The distribution of these growth factors in skin disease has not been determined. OBJECTIVE The purpose of this study was to determine the distribution of these growth factors in keratinocytic lesions and normal skin. METHODS Skin sections from common disorders of keratinocytes were examined by in situ hybridization with specific probes for aFGF, bFGF, and kFGF, and immunohistochemistry. RESULTS Of the growth factors studied, only bFGF was present in skin. bFGF messenger RNA was highly expressed in both normal epidermis and benign and malignant epithelial neoplasms. In normal skin, bFGF was expressed predominantly in a suprabasal fashion, whereas in epithelial neoplasms, homogeneous high-level expression of bFGF was observed. CONCLUSION bFGF is the primary member of the fibroblast growth factor expressed in the skin. The source of synthesis of bFGF is keratinocytes. Immunoreactivity for bFGF appears to be primarily confined to upper layers of the epidermis in normal skin, but is expressed at all layers of the epidermis in both benign and malignant neoplastic conditions. Genetic changes that promote epithelial tumors may also promote translation of bFGF messenger RNA into protein. Specific inhibition of bFGF activity may have application in the treatment of common skin diseases.