Controlled enzyme-catalyzed degradation of polymeric capsules templated on CaCO3: Influence of the number of LbL layers, conditions of degradation, and disassembly of multicompartments
Controlled enzyme-catalyzed degradation of polymeric capsules templated on CaCO3: Influence of the number of LbL layers, conditions of degradation, and disassembly of multicompartments
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DOI：
10.1016/j.jconrel.2012.08.006
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发表时间：
2012-09-28
影响因子：
10.8
通讯作者：
Skirtach, Andre
中科院分区：
文献类型：
--
作者：
Marchenko, Irina;Yashchenok, Alexey;Skirtach, Andre
提出了CaCO 3模板胶囊的酶催化降解。我们研究了a）生物可降解的，B）混合的生物可降解/合成的，和c）具有不同数量的聚合物层的多室多层胶囊。使用共聚焦激光扫描显微镜，我们观察到的非特异性蛋白酶链霉蛋白酶诱导的降解的胶囊的动力学减慢了几个小时的顺序，通过增加可生物降解的胶囊的壁中的层数，或通过插入合成的多层膜到壳组成的可生物降解的聚合物。链霉蛋白酶的浓度越高，降解速率越快。还显示了具有细胞内递送或体内应用潜力的多隔室胶囊的亚隔室的受控分离。(C)2012年爱思唯尔B。V.保留所有权利。
Enzyme-catalyzed degradation of CaCO3-templated capsules is presented. We investigate a) biodegradable, b) mixed biodegradable/synthetic, and c) multicompartment polyelectrolyte multilayer capsules with different numbers of polymer layers. Using confocal laser scanning microscopy we observed the kinetics of the non-specific protease Pronase-induced degradation of capsules is slowed down on the order of hours by either increasing the number of layers in the wall of biodegradable capsules, or by inserting synthetic polyelectrolyte multilayers into the shell comprised of biodegradable polymers. The degradation rate increases with the concentration of Pronase. Controlled detachment of subcompartments of multicompartment capsules, with potential for intracellular delivery or in-vivo applications, is also shown. (C) 2012 Elsevier B. V. All rights reserved.