Evidence for homologous peptidergic neurons in the buccal ganglia of diverse nudibranch mollusks.
Evidence for homologous peptidergic neurons in the buccal ganglia of diverse nudibranch mollusks.
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不同裸鳃类软体动物颊神经节中同源肽能神经元的证据。
DOI：
10.1002/neu.480230208
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Willows,AO
中科院分区：
文献类型：
--
作者：
Watson3rd,WH;Willows,AO
研究了7种裸科动物（乳头状小叶蝉、加利福尼亚阿米纳、斑点斑蝶、D. picta、Hermissenda crassicornis、Melibe leonina和tritonia diomedea）的颊神经节，以探索与针对小心脏活性肽B （SCPB）抗体反应的大细胞之间可能的同源性。每个物种的颊神经节都有一对大的、背侧的、白色的神经元，其中含有一种SCPB样肽。我们将这些神经元称为theSLB（SCPB免疫反应性大颊细胞）细胞。在所有被检查的物种中，SLB细胞突出胃食管神经，似乎支配食道。在每个物种中，通过对颊神经节分离制备的SLB神经元和其他神经元的细胞内记录观察到明显的节律性摄食运动程序（FMP）。SLB细胞通常以高频率放电，并且通常在与FMP活性相关的特定相位爆发。SLB细胞的刺激增强了进食运动程序的表达，通过增强现有的活性或在静止制剂中激发FMP。最后，在离体颊神经节中灌注scp6，激活SLB细胞并激活FMPs。因此，免疫组织化学和电生理数据都表明，在胆鳃目的三个亚目（树蕨科、银蕨科和紫蕨科）中，SLB细胞是同源的。我们的数据与先前发表的研究结果的比较表明，SLB细胞同源物可能也存在于其他腹足类动物中。
The buccal ganglia of seven nudibranches (Aeolidia papillosa, Armina californica, Dirona albolineata, D. picta, Hermissenda crassicornis, Melibe leonina, andTritonia diomedea) were examined to explore possible homologies between large cells that reacted with antibodies directed against small cardioactive peptide B (SCPB). The buccal ganglion of each species possessed a pair of large, dorsal–lateral, whitish neurons that contained an SCPB‐like peptide. We refer to these neurons as theSLB(SCPB‐immunoreactive Large Buccal) cells. In all species examined, the SLB cells project out the gastroesophageal nerves and appear to innervate the esophagus.In each species, an apparent rhythmic feeding motor program (FMP) was observed by intracellular recording from both SLB neurons and other neurons in isolated preparations of the buccal ganglia. SLB cells often fire at a high frequency, and usually burst in a specific phase relation to the FMP activity. Stimulation of SLB cells enhances expression of the feeding motor program, either by potentiating existing activity or eliciting the FMP in quiescent preparations. Finally, perfusion of isolated buccal ganglia with SCPBexcites the SLB cells and activates FMPs. Thus, both the immunohistochemical and electrophysiological data suggest that the SLB cells within three suborders of the opistobranchia (Dendronotacea, Arminacea, and Aeolidacea) are homologous. A comparison of our data with previously published studies indicates that SLB cell homologs may exist in other gastropods as well.