Collagen gel matrix assay as an in vitro chemosensitivity test for malignant astrocytic tumors
Collagen gel matrix assay as an in vitro chemosensitivity test for malignant astrocytic tumors
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DOI：
10.1007/s10147-006-0636-8
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发表时间：
2007-04-01
影响因子：
3.3
通讯作者：
Tanaka，Motohiro
中科院分区：
文献类型：
--
作者：
Ono，Atsushi;Kanno，Hiroshi;Tanaka，Motohiro
背景基于化疗敏感性的个体化化疗的疗效很少被研究。（4-氨基-2-甲基-5-嘧啶基）甲基-3（2-氯乙基）-3-亚硝基脲盐酸盐（ACNU），卡铂，顺铂，和足叶乙甙-43恶性星形细胞肿瘤（21例间变性星形细胞瘤和22例胶质母细胞瘤），并测定其生存期。结果顺铂、卡铂、ACNU和足叶乙甙对间变性星形细胞瘤的平均生长抑制率分别为33.2%、37.2%、28.0%和24.8%。在胶质母细胞瘤中，这些比率分别为36.9%、42.3%、23.2%和34.8%。接受过两种抗癌药物化疗的间变性星形细胞瘤患者的中位总体和无进展生存期（两种药物均显示出显著的抗癌活性（生长抑制率>30%））显著长于接受过两种药物治疗的患者，其中一种或两种药物均未显示出显著的抗癌活性。另一方面，有没有显着差异的整体或无进展生存在两个相应的组的胶质母细胞瘤轴承patients.ConclusionThe胶原凝胶基质测定是临床上有用的，以确定在体外化疗敏感性，反映在体内化疗敏感性。根据化疗敏感性数据，恶性星形细胞肿瘤的个体化化疗可能有助于延长生存期，特别是在间变性星形细胞瘤患者中。
BackgroundThe efficacy of individual chemotherapy based on chemosensitivity has scarcely been studied.MethodsWe examined the chemosensitivites for four anticancer agents – 1-(4-amino-2-methyl-5-pyrimidinyl) methyl-3 (2-chloroethyl)-3-nitrosourea hydrochloride (ACNU), carboplatin, cisplatin, and etoposide – of 43 malignant astrocytic tumors (21 anaplastic astrocytomas and 22 glioblastomas) by using a collagen gel matrix assay, and we also determined the survival periods of the tumor-bearing patients. The chemosensitivity was evaluated in terms of the growth inhibition rate, using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) method.ResultsFor the anaplastic astrocytomas, the mean growth inhibitory rate was 33.2% with cisplatin, 37.2% with carboplatin, 28.0% with ACNU, and 24.8% with etoposide. For the glioblastomas, these rates were 36.9%, 42.3%, 23.2%, and 34.8%, respectively. The median overall and progression-free survivals of anaplastic astrocytoma-bearing patients who had undergone chemotherapy with two anticancer drugs, both of which showed significant anticancer activity (growth inhibitory rate >30%) were significantly longer than those of the patients who had been treated with two drugs, one or both of which did not show significant anticancer activity. On the other hand, there was no significant difference in the overall or the progression-free survivals in the two corresponding groups of glioblastoma-bearing patients.ConclusionThe collagen gel matrix assay is clinically useful to determine in vitro chemosensitivity that reflects in vivo chemosensitivity. Individual chemotherapy for malignant astrocytic tumors, based on chemosensitivity data, could contribute to longer survival, particularly in anaplastic astrocytoma-bearing patients.