Epidermal papillomas and carcinomas induced in uninitiated mouse skin by tumor promoters alone contain a point mutation in the 61st codon of the Ha-ras oncogene.
Epidermal papillomas and carcinomas induced in uninitiated mouse skin by tumor promoters alone contain a point mutation in the 61st codon of the Ha-ras oncogene.
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仅由肿瘤启动子在未经启动的小鼠皮肤中诱导的表皮乳头状瘤和癌在 Ha-ras 癌基因的第 61 个密码子中含有点突变。
DOI：
10.1093/carcin/9.4.665
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发表时间：
1988
期刊：
影响因子：
4.7
通讯作者：
Strawhecker,J
中科院分区：
文献类型：
--
作者：
Pelling,JC;Neades,R;Strawhecker,J
以往多个实验室的研究结果表明，以7，12-二甲基苯并[a]菲(DMBA)为引发剂，两步法诱发的表皮乳头状瘤和癌组织中c-Ha-raso基因第61位密码子存在点突变。在本报告中，我们分析了从Sencar小鼠表皮中自发引发的乳头状瘤和癌症中提纯的DNA，这些肿瘤是在用促癌剂重复治疗后产生的。经限制性内切酶消化的肿瘤DNA的Southern杂交研究表明，9个乳头状瘤和癌中有7个在c-Ha-rasoncogene的一个等位基因第61位密码子上存在点突变。我们的发现对于点突变的Ha-ras原癌基因在启动皮肤肿瘤发生中所起的作用的意义进行了讨论。
Previous results in a number of laboratories have demonstrated that epidermal papillomas and carcinomas induced by the two-stage protocol of initiation and promotion contain a point mutation in the 61st codon of the c-Ha-rasoncogene when the initiating agent used is 7,12-dimethylbenz-[a]anthracene (DMBA). In the present report, we have analyzed DNA purified from ‘spontaneously initiated’ papillomas and carcinomas induced in SENCAR mouse epidermis after repetitive treatments with a tumor-promoting agent. Southern blot hybridization studies of tumor DNA digested with diagnostic restriction endonudeases demonstrated that seven of nine papillomas and carcinomas contained a point mutation in the 61st codon of one allele of the c-Ha-rasoncogene. The implications of our findings with respect to the role which a point-mutated Ha-rasprotooncogene plays in initiation of skin tumorigenesis are discussed.