Magneto-fluorescent nanoparticles with high-intensity NIR emission, T1-and T2-weighted MR for multimodal specific tumor imaging
Magneto-fluorescent nanoparticles with high-intensity NIR emission, T1-and T2-weighted MR for multimodal specific tumor imaging
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DOI：
10.1039/c5tb00155b
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发表时间：
2015-01-01
影响因子：
7
通讯作者：
Uvdal, Kajsa
中科院分区：
文献类型：
--
作者：
Wang, Guannan;Zhang, Xuanjun;Uvdal, Kajsa
合成了表现出明亮的近红外（NIR）荧光、T-1和T-2加权MR的纳米颗粒，用于特异性肿瘤成像。临床使用的Fe 3 O 4纳米颗粒表现出内在的暗信号（T2加权MRI），有时会误导临床诊断。在这里，我们第一次将超小的Fe 3 O 4纳米颗粒（2-3 nm）与发射NIR的半导体聚合物集成在一起，用于T-1和T-2加权MRI以及肿瘤的荧光成像。生物功能化的多模态荧光磁性纳米颗粒（FMNP）具有明亮的荧光和高弛豫（r（1）= 7.008 mM（-1）s（-1），r（2）= 26.788 mM（-1）s（-1），r（2）/r（1）= 3.8）。这些FMNP具有约20 nm的小平均动态尺寸，具有低聚集和长循环时间。体外研究表明，FMNP可以作为一种有效的荧光探针，实现对人肺癌A549细胞的靶向成像，且无明显的细胞毒性。体内实验结果表明，FMNP能够优先在肿瘤组织中积累，用于特异性荧光成像、T-1加权和T-2加权MRI。
Nanoparticles exhibiting bright near-infrared (NIR) fluorescence, T-1-and T-2-weighted MR were synthesized for specific tumor imaging. Clinically used Fe3O4 nanoparticles exhibit an intrinsic dark signal (T-2-weighted MRI), which sometimes misleads clinical diagnosis. Here, for the first time we integrated ultrasmall Fe3O4 nanoparticles (2-3 nm) with an NIR emitting semiconducting polymer for both T-1-and T-2-weighted MRI as well as fluorescence imaging of tumors. Bio-functionalized multi-modality fluorescent magnetic nanoparticles (FMNPs) functionalized with folic acid exhibit bright fluorescence and high relaxation (r(1) = 7.008 mM(-1) s(-1), r(2) = 26.788 mM(-1) s(-1), r(2)/r(1) = 3.8). These FMNPs have a small average dynamic size of about 20 nm with low aggregation and long circulation time. In vitro studies revealed that FMNPs can serve as an effective fluorescent probe to achieve targeting images of human A549 lung cancer cells without obvious cytotoxicity. In vivo experimental results show that the FMNPs are able to preferentially accumulate in tumor tissues for specific fluorescence imaging, T-1-and T-2-weighted MRI.