A note on the semi-quantitative estimation of aflatoxin M1 in liquid milk by thin-layer chromatography.
A note on the semi-quantitative estimation of aflatoxin M1 in liquid milk by thin-layer chromatography.
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关于薄层色谱法半定量测定液态奶中黄曲霉毒素 M1 的说明。
DOI：
10.1016/s0015-6264(68)80004-5
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发表时间：
1968
期刊：
影响因子：
--
通讯作者：
R. Allcroft
中科院分区：
文献类型：
--
作者：
B. A. Roberts;R. Allcroft
所描述的方法使用丙酮代替甲醇并确保令人满意的蛋白质沉淀和黄曲霉毒素提取。研究发现，500 公斤奶牛的牛奶中可识别黄曲霉毒素 M1 含量的 B1 每日最低摄入量约为 0.6-0.9 毫克。此时牛奶中黄曲霉毒素含量为0.0003 µg/ml，被认为是该方法鉴定的下限。
The method which is described uses acetone instead of methanol and ensures satisfactory precipitation of protein and extraction of aflatoxin. It was found that the lowest daily intake of B1 giving an identifiable amount of aflatoxin M1 in the milk of a 500-kg cow was of the order of 0.6-0.9 mg. At this intake aflatoxin content of the milk isc.0.0003 µg/ml which is considered as the lower limit for identification by this method.