Comparison of adventitious shoot formation from node and leaf explants of various carnation (Dianthus caryophyllus L.) cultivars
Comparison of adventitious shoot formation from node and leaf explants of various carnation (Dianthus caryophyllus L.) cultivars
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DOI：
10.1080/14620316.2002.11511532
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发表时间：
2002-01
期刊：
影响因子：
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通讯作者：
C. Nontaswatsri;S. Fukai;T. Touma;M. Goi
中科院分区：
文献类型：
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作者：
C. Nontaswatsri;S. Fukai;T. Touma;M. Goi
比较了35个康乃馨品种离体植株叶片外植体和38个温室植株节段外植体的茎再生情况。第一至第三节外植体在含1.0 mg l-1 TDZ的培养基上培养10年。在含1.0 mg l-1 BA的培养基上继代培养后，芽再生率较高，且上节的芽再生能力高于下节。相比之下，第1和第6离体叶片（在0.5 mg l-1 IBA和0.22 mg l-1 TDZ培养基上培养）的再生能力没有差异。茎节和叶外植体的再生能力因品种而异，标准型和喷型之间无差异。再生率与每再生节芽数、叶片外植体芽数呈极显著正相关（r=0.536**、0.720**，P<0.01）。茎节和叶外植体的茎再生率呈显著正相关（r=0.213*； P<0.05）。通过扫描电镜和光镜对不定芽器官发生的组织学观察表明，在节和叶外植体中，不定芽原基都是从叶基和茎之间的过渡区再生的。在节和叶外植体中，茎原基在创面附近的皮层组织中再生。
Summary Shoot regeneration of leaf explants from in vitro plants of 35 carnation cultivars, and node explants from greenhouse plants of 38 cultivars was compared. First to third node explants cultured on medium containing 1.0 mg l–1 TDZ for 10.d and subcultured onto medium contain 1.0 mg l–1 BA showed a high shoot regeneration percentage, while the upper node showed higher shoot regeneration ability than the lower node. In contrast, there was no difference in regeneration ability among the first to sixth in vitro leaves (cultured on medium with 0.5 mg l–1 IBA and 0.22 mg l–1 TDZ). In both node and leaf explants regeneration ability was different depending on the cultivar, and there was no difference between standard and spray types. The correlation coefficients between regeneration percentage and the number of shoots per regenerating node and leaf explants were significantly positive (r=0.536** and 0.720**, respectively P<0.01). The correlation coefficient between shoot regeneration percentage of node and leaf explants was significantly positive (r=0.213*; P<0.05). Histological observations on adventitious shoot organogenesis by SEM and light microscopy showed that in both node and leaf explants, shoot primordia were regenerated from the transition zone between the leaf base and the stem. In both node and leaf explants shoot primordia regenerated in the cortex tissue near the wound surface.