Deep Mutational Scanning: A Highly Parallel Method to Measure the Effects of Mutation on Protein Function.
Deep Mutational Scanning: A Highly Parallel Method to Measure the Effects of Mutation on Protein Function.
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DOI：
10.1101/pdb.top077503
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发表时间：
2015-08-03
影响因子：
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通讯作者：
Fields, Stanley
中科院分区：
文献类型：
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作者：
Starita, Lea M;Fields, Stanley
深度突变扫描是一种利用下一代测序技术在单个实验中测量蛋白质 10(5) 或更多独特变体的活性的方法。由于突变覆盖的深度，该策略提供了可分析的数据以揭示许多蛋白质特性。鉴于可用于酿酒酵母的广泛试剂和技术工具包，深度突变扫描方法特别适合在酿酒酵母中进行。
Deep mutational scanning is a method that makes use of next-generation sequencing technology to measure in a single experiment the activity of 10(5) or more unique variants of a protein. Because of this depth of mutational coverage, this strategy provides data that can be analyzed to reveal many protein properties. Deep mutational scanning approaches are particularly amenable to being performed in Saccharomyces cerevisiae, given the extensive toolkit of reagents and technologies available for this organism.