Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients.
Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients.
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DOI：
10.1038/ismej.2011.159
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发表时间：
2012-05
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影响因子：
--
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中科院分区：
文献类型：
--
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陆地生态系统正在接受来自人为来源的氮（N）输入，了解这些N可用性的增加如何影响土壤微生物群落对于预测地下生态系统的相关影响至关重要。我们使用了一套方法来分析土壤微生物群落的结构和功能特征，从两个长期定位在对比系统中的氮肥施肥试验的重复样地。基于Pyrosequencing的16 S rRNA基因的分析显示，氮施肥对细菌多样性没有显着影响，但在这两个网站的社区组成的显着影响;共生类群（包括变形菌门和拟杆菌门的成员）通常增加相对丰度在高N地块，寡营养类群（主要是酸杆菌）表现出相反的模式。与氮施肥下的系统发育变化一致，鸟枪法宏基因组测序显示与DNA/RNA复制，电子传递和蛋白质代谢相关的基因的相对丰度增加，即使在这里进行的浅鸟枪法宏基因组测序（平均每个样品75 000个读数）也可以解决。我们还观察到在整个N梯度的社区，显着相关的系统发育和宏基因组响应的分解代谢能力的变化，表明土壤微生物群落的结构和功能之间可能存在联系。总的来说，我们的研究结果表明，N施肥可能会直接或间接地引起主要微生物生活史策略的转变，有利于更活跃的共生微生物群落，这种模式与经常观察到的K-选择与R-选择植物物种的替代具有升高的N。
Terrestrial ecosystems are receiving elevated inputs of nitrogen (N) from anthropogenic sources and understanding how these increases in N availability affect soil microbial communities is critical for predicting the associated effects on belowground ecosystems. We used a suite of approaches to analyze the structure and functional characteristics of soil microbial communities from replicated plots in two long-term N fertilization experiments located in contrasting systems. Pyrosequencing-based analyses of 16S rRNA genes revealed no significant effects of N fertilization on bacterial diversity, but significant effects on community composition at both sites; copiotrophic taxa (including members of the Proteobacteria and Bacteroidetes phyla) typically increased in relative abundance in the high N plots, with oligotrophic taxa (mainly Acidobacteria) exhibiting the opposite pattern. Consistent with the phylogenetic shifts under N fertilization, shotgun metagenomic sequencing revealed increases in the relative abundances of genes associated with DNA/RNA replication, electron transport and protein metabolism, increases that could be resolved even with the shallow shotgun metagenomic sequencing conducted here (average of 75 000 reads per sample). We also observed shifts in the catabolic capabilities of the communities across the N gradients that were significantly correlated with the phylogenetic and metagenomic responses, indicating possible linkages between the structure and functioning of soil microbial communities. Overall, our results suggest that N fertilization may, directly or indirectly, induce a shift in the predominant microbial life-history strategies, favoring a more active, copiotrophic microbial community, a pattern that parallels the often observed replacement of K-selected with r-selected plant species with elevated N.
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DOI：
10.1073/pnas.0409727102
发表时间：
2005-02-15
影响因子：
11.1
作者：
Konstantinidis, KT;Tiedje, JM
通讯作者：
Tiedje, JM
影响因子：
4.8
作者：
Fierer, Noah;Bradford, Mark A.;Jackson, Robert B.
通讯作者：
Jackson, Robert B.
影响因子：
3.7
作者：
Frey, SD;Knorr, M;Simpson, RT
通讯作者：
Simpson, RT
影响因子：
8.8
作者：
Fukami, Tadashi;Dickie, Ian A.;Allen, Robert B.
通讯作者：
Allen, Robert B.
影响因子：
9.7
作者：
Degens, BP;Schipper, LA;Vojvodic-Vukovic, M
通讯作者：
Vojvodic-Vukovic, M