Expression of type IV collagen and laminin at the interface between epithelial cells and fibroblasts from human periodontal ligament
Expression of type IV collagen and laminin at the interface between epithelial cells and fibroblasts from human periodontal ligament
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DOI：
10.1111/j.1600-0722.2004.00178.x
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发表时间：
2005-02-01
影响因子：
1.9
通讯作者：
Komatsu, M
中科院分区：
文献类型：
--
作者：
Shimonishi, M;Sato, J;Komatsu, M
本研究旨在研究Malassez上皮细胞（ERM）周围IV型胶原和层粘连蛋白的合成是否需要ERM细胞和牙周韧带成纤维细胞直接接触。人牙周韧带（HPDL）外植体在改良的无血清培养基中培养时产生含有ERM细胞和成纤维细胞的外植体。采用相衬显微镜（PCM）和扫描电镜（SEM）观察ERM细胞与成纤维细胞之间的界面。采用免疫组织化学和原位杂交技术研究IV型胶原和层粘连蛋白的表达。观察到ERM细胞生长在成纤维细胞下方或附着于成纤维细胞上。在界面处，IV型胶原蛋白和层粘连蛋白及其各自的mrna在ERM细胞和成纤维细胞中都很丰富，而这些蛋白和mrna在远离界面的细胞中几乎没有染色。因此，这些发现表明，IV型胶原和层粘连蛋白的合成是由ERM细胞和牙周韧带成纤维细胞之间的直接相互作用诱导的。
The present study was undertaken to examine whether synthesis of type IV collagen and laminin around the epithelial rests of Malassez (ERM) requires direct contact between cells from ERM and periodontal ligament fibroblasts. Human periodontal ligament (HPDL) explants produced outgrowths containing both ERM cells and fibroblasts when cultured in a modified serum-free medium. The interface between ERM cells and fibroblasts was examined using phase-contrast microscopy (PCM) and scanning electron microscopy (SEM). Expression of type IV collagen and laminin was studied by immunohistochemistry and in situ hybridization. It was observed that ERM cells grew underneath fibroblasts or attached to them. At the interface, type IV collagen and laminin and their respective mRNAs were abundant in both ERM cells and fibroblasts, while these proteins and mRNAs showed little if any staining in cells further away from the interface. Hence, these findings indicate that synthesis of type IV collagen and laminin is induced by direct interaction between ERM cells and periodontal ligament fibroblasts.