Group-specific structural features of the 5′-proximal sequences of coronavirus genomic RNAs
Group-specific structural features of the 5′-proximal sequences of coronavirus genomic RNAs
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DOI：
10.1016/j.virol.2010.02.007
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发表时间：
2010-05-25
期刊：
影响因子：
3.7
通讯作者：
Olsthoorn, Rene C. L.
中科院分区：
文献类型：
--
作者：
Chen, Shih-Cheng;Olsthoorn, Rene C. L.
对冠状病毒(Coy)5‘非翻译区和相邻编码序列二级结构的全局预测表明，存在保守的结构元件。在所有冠状病毒中都预测到了茎环(SL)1、2、4和5，而核心前导转录调节序列(L-TRS)只在部分CoV中形成了SL3。除HA CoV外，I组和II组CoV中的SL5的特征是存在能够与保守的5‘-UUYCGU-3’环序列形成发夹的大序列插入。结构探测证实了这些发夹在I组人类冠状病毒229E和II组严重急性呼吸综合征冠状病毒(SARS-CoV)中的存在。总体而言，5‘端顺式作用元件的模式与CoV的谱系高度相关，包括SL5中保守的发夹的特征。这些保守的发夹作为假定的包装信号的功能进行了讨论。(C)2010 Elsevier Inc.保留所有权利。
Global predictions of the secondary structure of coronavirus (Coy) 5 ' untranslated regions and adjacent coding sequences revealed the presence of conserved structural elements. Stem loops (SL) 1, 2, 4, and 5 were predicted in all CoVs, while the core leader transcription-regulating sequence (L-TRS) forms SL3 in only some CoVs. SL5 in group I and II CoVs, with the exception of group ha CoVs, is characterized by the presence of a large sequence insertion capable of forming hairpins with the conserved 5'-UUYCGU-3' loop sequence. Structure probing confirmed the existence of these hairpins in the group I Human coronavirus-229E and the group II Severe acute respiratory syndrome coronavirus (SARS-CoV). In general, the pattern of the 5' cis-acting elements is highly related to the lineage of CoVs, including features of the conserved hairpins in SL5. The function of these conserved hairpins as a putative packaging signal is discussed. (C) 2010 Elsevier Inc. All rights reserved.