Rapid method for direct identification of bacteria in urine and blood culture samples by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: intact cell vs. extraction method
Rapid method for direct identification of bacteria in urine and blood culture samples by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: intact cell vs. extraction method
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DOI：
10.1111/j.1469-0691.2010.03339.x
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发表时间：
2011-07-01
影响因子：
14.2
通讯作者：
Gonzalez-Buitrago, J. M.
中科院分区：
文献类型：
--
作者：
Ferreira, L.;Sanchez-Juanes, F.;Gonzalez-Buitrago, J. M.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）是一种快速、可靠的微生物蛋白质组学鉴定技术。在这里，我们比较了完整的细胞方法和蛋白质提取方法，然后应用于MALDI板上的尿液和血液培养样品中的微生物从临床微生物实验室的直接鉴定。结果表明，完整细胞法为尿液提供了极好的结果，是血培养的良好初始方法。该提取方法补充了完整细胞法，改善了血培养中微生物的鉴定。因此，我们认为，MALDI-TOF MS直接进行尿液和血液培养样本，与我们提出的协议，是一个合适的技术微生物鉴定，在临床微生物实验室中使用的常规方法相比。
Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is a fast and reliable technology for the identification of microorganisms with proteomics approaches. Here, we compare an intact cell method and a protein extraction method before application on the MALDI plate for the direct identification of microorganisms in both urine and blood culture samples from clinical microbiology laboratories. The results show that the intact cell method provides excellent results for urine and is a good initial method for blood cultures. The extraction method complements the intact cell method, improving microorganism identification from blood culture. Thus, we consider that MALDI-TOF MS performed directly on urine and blood culture samples, with the protocols that we propose, is a suitable technique for microorganism identification, as compared with the routine methods used in the clinical microbiology laboratory.