Transmission of binding information across lipid bilayers
Transmission of binding information across lipid bilayers
复制标题
DOI：
10.1002/chem.200601723
复制
发表时间：
2007-01-01
影响因子：
4.3
通讯作者：
Williams, Nicholas H.
中科院分区：
文献类型：
--
作者：
Dijkstra, Harmen P.;Hutchinson, Jordan J.;Williams, Nicholas H.
报道了一种能够跨脂质双层膜传递结合信息的合成跨膜受体。结合事件是基于铜（II）络合乙二胺功能触发的受体的聚集。通过用荧光丹磺酰基基团标记受体，可以通过测量荧光猝灭的程度来监测铜（II）结合事件。将受体与缺乏跨膜连接的对照受体进行比较发现，在低铜（II）浓度下，跨膜受体比非跨越对照受体更紧密地结合铜（II）离子。由于两种受体与铜（II）的内在结合是相同的，因此这种效应归因于跨膜受体连接的内部和外部结合侧之间的协同作用。因此，这是第一次报道的人工信号事件，其中膜一侧的信使结合导致膜另一侧的合作结合事件，类似于生物信号系统，并帮助我们更好地了解更有效的人工信号系统的要求。
A synthetic transmembrane receptor that is capable of transmitting binding information across a lipid bilayer membrane is reported. The binding event is based on aggregation of the receptor triggered by copper(II) complexation to ethylenediamine functionalities. By labelling the receptor with fluorescent dansyl groups, the copper(II) binding event could be monitored by measuring the extent of fluorescence quenching. Comparing the receptor with a control receptor lacking the transmembrane linkage revealed that the transmembrane receptor binds copper(II) ions more tightly than the non-spanning control receptor at low copper(II) concentrations. Since the intrinsic binding to copper(II) is the same for both receptors, this effect was attributed to synergy between the connected interior and exterior binding sides of the transmembrane receptor. Thus, this is the first reported artificial signalling event in which binding of a messenger on one side of the membrane leads to a cooperative binding event on the opposite side of the membrane, resembling biological signalling systems and helping us to get a better understanding of the requirements for more effective artificial signalling systems.