Mice immune responses to Brucella abortus heat shock proteins -: Use of baculovirus recombinant-expressing whole insect cells, purified Brucella abortus recombinant proteins, and a vaccinia virus recombinant as immunogens
Mice immune responses to Brucella abortus heat shock proteins -: Use of baculovirus recombinant-expressing whole insect cells, purified Brucella abortus recombinant proteins, and a vaccinia virus recombinant as immunogens
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DOI：
10.1016/s0378-1135(02)00101-3
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发表时间：
2002-08-25
影响因子：
3.3
通讯作者：
Toth, TE
中科院分区：
文献类型：
--
作者：
Bae, JE;Schurig, GG;Toth, TE
流产布鲁氏菌抵抗巨噬细胞的杀菌机制，其热休克蛋白（HSP）如GroEL、GroES和HtrA的表达可能在这种抵抗中发挥作用。细菌热休克蛋白具有很强的免疫原性，可在各种类型的细菌感染中诱导保护性免疫。然而，针对流产布鲁氏菌热休克蛋白的免疫反应在预防布鲁氏菌病中的重要性目前尚未解决。为了阐明这些蛋白质在抵御布鲁氏菌攻击中的作用，将流产布鲁氏菌 GroEL、GroES 和/或 HtrA 的个体、二价或三价杆状病毒 (BV) 重组体作为表达蛋白质的全细胞或作为纯化蛋白质注射到 BALB/c 小鼠中。将这些制剂分别与弗氏佐剂或里比佐剂联合给予小鼠。此外，一些小鼠用痘苗病毒-GroEL 重组体进行免疫，然后接种纯化的 GroEL-Ribi 佐剂组合。观察到针对流产布鲁氏菌 GroEL 和 HtrA 的抗体，但未观察到针对 GroES 的抗体。通过观察用纯化的 HtrA-Ribi 佐剂组合免疫的小鼠淋巴细胞显着释放 IFN-γ，证明了细胞免疫反应。然而，接种单独的二价或三价HSP表达细胞与完全弗氏佐剂组合的小鼠或接种纯化的流产布鲁氏菌HSP与Ribi佐剂组合的小鼠均未受到保护以免受流产布鲁氏菌强毒株2308的攻击。用牛痘病毒-GroEL重组体初免并用GroEL-Ribi组合加强并没有诱导保护性免疫。根据所获得的结果，我们认为，虽然流产布鲁氏菌HSP诱导了体液和细胞介导的免疫反应，但保护性免疫反应并未诱导。 (C) 2002 Elsevier Science B.V. 保留所有权利。
Brucella abortus resists the microbicidal mechanisms of macrophages, and the expression of its heat shock proteins (HSPs) such as GroEL, GroES and HtrA may play a role in this resistance. Bacterial HSPs can be very immunogenic, inducing protective immunity in various types of bacterial infections. However, the significance of immune responses directed against B. abortus HSPs in the protection against brucellosis is currently unresolved. To elucidate the role of these proteins in protection against Brucella challenge, individual, divalent or trivalent baculovirus (BV) recombinants of B. abortus GroEL, GroES and/or HtrA were injected into BALB/c mice either as protein-expressing whole cells or as purified proteins. The preparations were given to mice in combination with Freund's or Ribi adjuvant, respectively. In addition, some mice were primed with a vaccinia virus-GroEL recombinant, followed by inoculation with purified GroEL-Ribi adjuvant combination. Antibodies were observed against B. abortus GroEL and HtrA, but not against GroES. Cellular immune response was demonstrated by observing significant IFN-gamma release by lymphocytes of mice immunized with the purified HtrA-Ribi adjuvant combination. However, none of the mice inoculated with individual, divalent or trivalent HSP-expressing cells combined with complete Freund's adjuvant or inoculated with purified B. abortus HSPs combined with Ribi adjuvant, were protected against challenge with B. abortus virulent strain 2308. Priming with vaccinia virus-GroEL recombinant and boosting with GroEL-Ribi combination did not induce protective immunity. Based on the results obtained, we suggest that although humoral and cell-mediated immune responses are induced, but protective immune response is not induced by B. abortus HSPs. (C) 2002 Elsevier Science B.V. All rights reserved.