Regulation of carotenoid and ABA accumulation during the development and germination of Nicotiana plumbaginifolia seeds
Regulation of carotenoid and ABA accumulation during the development and germination of Nicotiana plumbaginifolia seeds
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DOI：
10.1007/s00425-006-0231-2
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发表时间：
2006-08-01
期刊：
影响因子：
4.3
通讯作者：
Marion-Poll, Annie
中科院分区：
文献类型：
--
作者：
Frey, Anne;Boutin, Jean-Pierre;Marion-Poll, Annie
脱落酸（阿坝）是由环氧类胡萝卜素裂解产生的，调节种子的发育和成熟。通过类胡萝卜素分析，研究了类胡萝卜素合成对烟草种子发育过程中阿坝积累的调控作用。叶黄素的最大积累发生在野生型种子的中期发展，当总阿坝水平也达到峰值。与此相反，在ABA缺陷突变体叶黄素合成延迟，在协议的延迟种子成熟。在玉米黄质环氧化酶（ZEP）将玉米黄质转化为紫黄质的突变体中，通过在种子发育后期诱导表达的启动子控制下引入拟南芥AtZEP基因，与野生型NpZEP相比，以及在干燥和吸胀的种子中，种子休眠得到恢复。ZEP表达的时间和水平的改变并没有高度影响转基因种子中阿坝积累的时间调节，尽管叶黄素积累有显着的扰动。因此，阿坝积累的主要调控可能发生在环氧类胡萝卜素合成的下游。
Abscisic acid (ABA) is derived from epoxycarotenoid cleavage and regulates seed development and maturation. A detailed carotenoid analysis was undertaken to study the contribution of epoxycarotenoid synthesis to the regulation of ABA accumulation in Nicotiana plumbaginifolia developing seeds. Maximal accumulation of xanthophylls occurred at mid-development in wild type seeds, when total ABA levels also peaked. In contrast, in ABA-deficient mutants xanthophyll synthesis was delayed, in agreement with the retardation in seed maturation. Seed dormancy was restored in mutants impaired in the conversion of zeaxanthin into violaxanthin by zeaxanthin epoxidase (ZEP), by the introduction of the Arabidopsis AtZEP gene under the control of promoters inducing expression during later stages of seed development compared to wild type NpZEP, and in dry and imbibed seeds. Alterations in the timing and level of ZEP expression did not highly affect the temporal regulation of ABA accumulation in transgenic seeds, despite notable perturbations in xanthophyll accumulation. Therefore, major regulatory control of ABA accumulation might occur downstream of epoxycarotenoid synthesis.