Dye diffusion at surfaces: charge matters.
Dye diffusion at surfaces: charge matters.
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DOI：
10.1021/la904749z
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发表时间：
2010-04-06
期刊：
影响因子：
--
通讯作者：
Landes CF
中科院分区：
文献类型：
--
作者：
Daniels CR;Reznik C;Landes CF
荧光相关光谱和单分子爆发分析被用来测量玻璃表面相互作用对两种常见的荧光染料，一种阳离子和一种阴离子的扩散的影响。染料表面相互作用的影响，作为从表面的距离的函数测量的扩散速率进行了研究。使用三轴压电平台，结合参考校准测量，能够准确获取表面距离相关的传输数据。该分析揭示了阳离子染料和表面之间的吸引力相互作用，这显著改变了提取的扩散值，并在距离表面高达1.0 μm的测量中持续存在。阳离子染料和表面之间的库仑吸引力也导致罕见的、长寿命的缔合事件，其导致提取的扩散值的不可再现性。除了这些事件的关联寿命的分配，本文表明，如果实验必须与阳离子探针附近的玻璃表面进行，使用溶液电解质可以消除有害的染料表面相互作用，作为染料在各种环境中进行了测试。最后，我们的数据表明，一个更好的染料选择是阴离子探针，它表现出没有深度依赖性的扩散特性以上的玻璃表面。
Fluorescence correlation spectroscopy and single molecule burst analysis were used to measure the effects of glass surface interactions on the diffusion of two common fluorescent dyes, one cationic and one anionic. The effects of dye–surface interactions on measured diffusion rates as a function of distance from the surface were investigated. Use of a three-axis piezo stage, combined with reference calibration measurements, enabled the accurate acquisition of surface-distance-dependent transport data. This analysis reveals attractive interactions between the cationic dye and the surface, which significantly alter the extracted diffusion values and persist in the measurements up to 1.0 μm from the surface. The Coulomb attraction between the cationic dye and the surface also results in rare, long-lived association events that lead to irreproducibility in extracted diffusion values. In addition to an assignment of the association lifetime for these events, this paper demonstrates that, if experiments must be performed with cationic probes near a glass surface, the use of solution electrolytes can eliminate deleterious dye–surface interactions, as the dyes were tested in a variety of environments. Finally, our data demonstrate that a better dye choice is an anionic probe, which exhibits no depth dependence of diffusion characteristics above a glass surface.
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DOI：
10.1073/pnas.95.4.1556
发表时间：
1998-02-17
影响因子：
11.1
作者：
Eggeling, C;Fries, JR;Seidel, CAM
通讯作者：
Seidel, CAM
影响因子：
2.8
作者：
Loman, Anastasia;Dertinger, Thomas;Enderlein, Joerg
通讯作者：
Enderlein, Joerg
影响因子：
2.9
作者：
Dertinger, Thomas;Pacheco, Victor;Enderlein, Joerg
通讯作者：
Enderlein, Joerg
影响因子：
56.9
作者：
BEDZYK, MJ;BOMMARITO, GM;PENNER, TL
通讯作者：
PENNER, TL
影响因子：
3.9
作者：
Helt, JM;Batteas, JD
通讯作者：
Batteas, JD