Identification of sn-glycerol-1-phosphate dehydrogenase activity from genomic information on a hyperthermophilic archaeon, Sulfolobus tokodaii Strain 7
Identification of sn-glycerol-1-phosphate dehydrogenase activity from genomic information on a hyperthermophilic archaeon, Sulfolobus tokodaii Strain 7
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DOI：
10.1271/bbb.70.282
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发表时间：
2006-01-01
影响因子：
1.6
通讯作者：
Kawarabayasi, Y
中科院分区：
文献类型：
--
作者：
Koga, Y;Ohga, M;Kawarabayasi, Y
sn-甘油-1-磷酸脱氢酶负责sn-甘油-1-磷酸的形成，sn-甘油-1-磷酸是海藻膜磷脂的骨架。这种活性从未在硫化叶菌的无细胞提取物中检测到。在这里，我们报告了在大肠杆菌中表达的硫化叶菌tokodaii的热稳定性ST 0344蛋白上检测到这种活性，这是从S. tokodaii。这是该酶形成sn-甘油-1-磷酸的一般机制的另一条证据。
sn-Glycerol-1-phosphate dehydrogenase is responsible for the formation of sn-glycerol-1-phosphate, the backbone of membrane phospholipids of Archaea. This activity had never been detected in cell-free extract of Sulfolobus sp. Here we report the detection of this activity on the thermostable ST0344 protein of Sulfolobus tokodaii expressed in Escherichia coli, which was predicted from genomic information on S. tokodaii. This is another line of evidence for the general mechanism of sn-glycerol-1-phosphate formation by the enzyme.