Interaction of the nuclear protein CBF1 with the κB Site of the IL-6 gene promoter
Interaction of the nuclear protein CBF1 with the κB Site of the IL-6 gene promoter
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DOI：
10.1093/nar/27.13.2785
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发表时间：
1999-07-01
影响因子：
14.9
通讯作者：
Furia, A
中科院分区：
文献类型：
--
作者：
Palmieri, M;Sasso, MP;Furia, A
核蛋白CBF 1是一种将转录因子（如活化的Notch受体）靶向至特定DNA位点的中间体。本文中，我们证明了来自不同来源的细胞系的CBF 1能够与IL-6启动子的κ B位点结合。通过在HeLa细胞中进行的转染分析，我们证明，过表达的CBF 1作为IL-6基因转录的负调节因子，并且不能引起该基因的Notch依赖性激活。对蛋白质-DNA相互作用的分析表明，CBF 1和靶DNA形成的复合物的拓扑结构受到识别位点周围序列的微妙影响。此外，我们表明，CBF 1诱导DNA弯曲。这一发现表明，CBF 1可能影响IL-6基因的转录，通过确定启动子区的特定构象。
The nuclear protein CBF1 has been shown to function as an intermediate to target transcription factors, such as the activated Notch receptor, to specific DNA sites, In this paper, we show that CBF1 from cell lines of different origin is able to bind to the kappa B site Of the IL-6 promoter. By transfection analyses performed in HeLa cells, we demonstrate that overexpressed CBF1 acts as a negative regulator of IL-6 gene transcription and is unable to elicit Notch-dependent activation of this gene. Analyses of protein-DNA interactions indicate that the topology of the complex formed by CBF1 and the target DNA is subtly affected by sequences surrounding the recognition site. Furthermore, we show that CBF1 induces DNA bending. This finding suggests that CBF1 may influence IL-6 gene transcription by determining a specific conformation of the promoter region.