Increase in complement component C3 is an early response to experimental magnesium deficiency in rats
Increase in complement component C3 is an early response to experimental magnesium deficiency in rats
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DOI：
10.1016/s0024-3205(03)00291-1
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发表时间：
2003-06-13
期刊：
影响因子：
6.1
通讯作者：
Rayssiguier, Y
中科院分区：
文献类型：
--
作者：
Bussière, FI;Tridon, A;Rayssiguier, Y
炎症过程在实验性镁缺乏症病理学中的重要性已被重新考虑，但导致炎症反应的事件顺序仍不清楚。本研究通过测定大鼠肝脏总补体C_3浓度、RT-PCR法测定大鼠肝脏补体前原C_3 mRNA丰度、Western Blot法测定大鼠补体溶血活性和C_3活化，研究了缺镁对补体系统的影响。断奶雄性Wistar大鼠喂养镁缺乏或对照实验饮食2或8天。在第8天，镁缺乏症的特征性炎症反应，包括充血，白细胞增多和脾脏肿大，伴随着血浆中总C3量的增加。此外，在第8天，RT-PCR分析表明，与对照组大鼠相比，Mg缺乏大鼠肝脏中大鼠前原补体C3的mRNA水平更高。即使2天后在大鼠中未观察到炎性综合征，与对照大鼠中的总血浆C3水平相比，总血浆C3显示出显著增加。由于Wistar大鼠中补体溶血活性值的高度变异性，在第二项实验中使用了断奶雄性Sprague-Dawley大鼠。在第8天，Sprague-Dawley大鼠的炎症反应伴随着总C3量的增加和更高的溶血活性。蛋白质印迹技术未能显示明显的条带，从镁缺乏大鼠血浆中C3裂解。由于补体C3是阳性急性期反应物，C3的升高表明炎症反应的改变是Mg缺乏的早期事件。然而，补体激活似乎并不参与急性期的缺乏。(C)2003年爱思唯尔科学公司All rights reserved.
The importance of the inflammatory process in the pathology of experimental Mg-deficiency has been reconsidered but the sequence of events leading to inflammatory response remains unclear. In this study, the effect of Mg-deficiency on complement system by measuring total C3 concentration, mRNA abundance for rat pre-pro complement C3 in liver by RT-PCR, complement haemolytic activity and C3 activation by Western Blot was studied. Weaning male Wistar rats were fed either Mg-deficient or control experimental diets for 2 or 8 days. At 8 days, a characteristic inflammatory response of Mg-deficiency including hyperaemia, leukocytosis and enlarged spleen was accompanied by an increase in the total C3 quantity in plasma. Moreover, at 8 days, RT-PCR analysis indicated higher level of mRNA rat pre-pro complement C3 in liver from Mg-deficient rats compared to control rats. Even if the inflammatory syndrome was not observed in rats after 2 days, total plasma C3 was shown to be significantly increased as compared to total plasma C3 level in control rats. Because of the high variability of complement haemolytic activity values in Wistar rats, weaning male Sprague-Dawley rats were used in a second experiment. At 8 days, the inflammatory response of Sprague-Dawley rats was accompanied by an increase in total C3 quantity and by a higher haemolytic activity. The Western Blot technique failed to display distinct bands resulting from C3 cleavage in plasma from Mg-deficient rats. Since, the complement C3 is a positive acute phase reactant, the elevation of C3 indicates that the modification of inflammatory response is an early event of Mg-deficiency. However, complement activation does not appear to be involved in the acute phase of the deficiency. (C) 2003 Elsevier Science Inc. All rights reserved.