Video imaging of walking myosin V by high-speed atomic force microscopy
Video imaging of walking myosin V by high-speed atomic force microscopy
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DOI：
10.1038/nature09450
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发表时间：
2010-11-04
期刊：
影响因子：
64.8
通讯作者：
Ando, Toshio
中科院分区：
文献类型：
--
作者：
Kodera, Noriyuki;Yamamoto, Daisuke;Ando, Toshio
肌球蛋白V分子沿沿着肌动蛋白丝移位的动力学行为主要用光学显微镜研究。由此证明了与三磷酸腺苷水解相结合的进行性手-手运动。然而，蛋白质分子本身在观察中是不可见的，因此在稳态下通过电子显微镜可见。伴随的结构和动力学的评估一直是不可行的，在整个生物学研究中普遍存在的情况。在这里，我们直接可视化肌球蛋白V分子走沿着肌动蛋白轨道，使用高速原子力显微镜。高分辨率电影不仅为先前推测或证明的分子行为（包括双臂摆动）提供了确凿的“视觉证据”，而且还揭示了分子的更详细行为，从而全面了解运动机制。我们的直接和动态的高分辨率可视化是一种强大的新方法来研究生物分子的结构和动力学。
The dynamic behaviour of myosin V molecules translocating along actin filaments has been mainly studied by optical microscopy. The processive hand-over-hand movement coupled with hydrolysis of adenosine triphosphate was thereby demonstrated. However, the protein molecules themselves are invisible in the observations and have therefore been visualized by electron microscopy in the stationary states. The concomitant assessment of structure and dynamics has been unfeasible, a situation prevailing throughout biological research. Here we directly visualize myosin V molecules walking along actin tracks, using high-speed atomic force microscopy. The high-resolution movies not only provide corroborative 'visual evidence' for previously speculated or demonstrated molecular behaviours, including lever-arm swing, but also reveal more detailed behaviours of the molecules, leading to a comprehensive understanding of the motor mechanism. Our direct and dynamic high-resolution visualization is a powerful new approach to studying the structure and dynamics of biomolecules in action.