Prevalence of Epstein-Barr virus infection and characteristics of lymphocyte subsets in newly onset juvenile dermatomyositis
Prevalence of Epstein-Barr virus infection and characteristics of lymphocyte subsets in newly onset juvenile dermatomyositis
复制标题
DOI：
10.1007/s12519-019-00314-7
复制
发表时间：
2021-04-01
影响因子：
8.7
通讯作者：
Lu, Mei-Ping
中科院分区：
文献类型：
--
作者：
Zheng, Qi;Zhu, Kun;Lu, Mei-Ping
背景幼年型皮肌炎(JDM)的潜在病因尚不清楚。T细胞缺陷和EB病毒(EBV)感染被怀疑参与了JDM的发病机制，但在JDM患者中的评估很差。方法观察初发JDM患者T、B淋巴细胞亚群的特点，并与正常对照组比较，分析JDM患者EBV感染情况。2014年至2018年新发现的JDM患者包括在研究中。于入院时及治疗6个月后用流式细胞仪检测不同标志物(CD3(+)、CD3(+)、CD4(+)、CD3(+)、CD8(+)、CD3(-)、CD19(+)、CD3(-)、CD16(+)、CD56(+))的淋巴细胞。对JDM组和对照组的EBV感染情况进行统计学分析。结果JDM组EB病毒衣壳抗原阳性率和EB病毒衣壳抗原阳性率均高于正常对照组(P<0.0001)。JDM患者早期CD3(-)、CD16(+)、CD56(+)淋巴细胞极低，治疗6个月后升高(P=0.0091)。结论CD3(-)、CD16(+)、CD56(+)细胞水平可能与JDM的临床病程有关。EBV可能是JDM发病的环境因素。
Background The underlying etiology of juvenile dermatomyositis (JDM) is unknown. T cell deficiency as well as Epstein-Barr virus (EBV) infection had been suspected to be involved in the pathogenesis, but it has been poorly evaluated in JDM patients. Methods This study described the traits of T and B lymphocyte subsets in newly onset JDM patients and the incidence of EBV infection in JDM patients compared with match controls. Newly developed JDM patients from 2014 to 2018 were included in the study. Lymphocytes with different markers (CD3(+), CD3(+)CD4(+), CD3(+)CD8(+), CD3(-)CD19(+) and CD3(-)CD16(+)CD56(+)) were tested with flow cytometry in the first admission or after 6 months of treatment. Statistical analysis was conducted to compare the EBV infection in the group of JDM patients and controls. Results We observed that JDM patients had higher positive rate of Epstein-Barr nuclear antigen-immunoglobulin G (IgG) (P < 0.0001) as well as EBV capsid antigen-IgG (P < 0.05) than normal controls. CD3(-)CD16(+)CD56(+) lymphocyte was found to be extremely low in early stage of JDM patients, but increased after 6 months of treatment (P = 0.0091). Conclusions The level of CD3(-)CD16(+)CD56(+) cells may associate with the clinical course of JDM. EBV may act as an environmental factor predisposing patients to the development of JDM.