Sublethal effects of halofenozide on larval development and detoxification in Phaedon brassicae (Coleoptera: Chrysomelidae)
Sublethal effects of halofenozide on larval development and detoxification in Phaedon brassicae (Coleoptera: Chrysomelidae)
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DOI：
10.1093/jee/toad113
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发表时间：
2023-06
影响因子：
2.2
通讯作者：
Long Ma;Changxia Xu;Yingchuan Peng;Jing Zhang;Wanna Zhang
中科院分区：
文献类型：
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作者：
Long Ma;Changxia Xu;Yingchuan Peng;Jing Zhang;Wanna Zhang
芸苔叶甲（Phaedon brassicae）是十字花科作物的严重食叶害虫。氟虫酰肼（Halofenozide，Hal）是一种蜕皮激素激动剂，是一类新型的昆虫生长调节杀虫剂。初步试验表明，Hal对甘蓝夜蛾幼虫具有显著的毒力。然而，这种化合物在昆虫中的代谢降解仍不清楚。在这项研究中，口服给药的Hal在LC 10和LC 25造成严重的角质层和表皮分离，导致幼虫蜕皮失败。亚致死剂量的暴露也显着降低幼虫的呼吸速率，以及他们的化蛹率和蛹重。相反，多功能氧化酶，羧酸酯酶（CarE），谷胱甘肽S-转移酶（GST）的活性显着增强在哈尔处理的幼虫。利用RNA测序进一步分析鉴定了64个差异表达的解毒酶基因，包括31个P450，13个GST和20个CarE。在25个上调的P450基因中，有22个基因属于CYP 3家族，另外3个基因属于CYP 4家族。同时，3 sigma类GST和7 sigma类GST显著增加，占上调GST的大多数。此外，18个过表达的CarEs中有16个聚在鞘翅目异源物质代谢组中。这些结果表明，在暴露于亚致死剂量的Hal后，P. brassicae的解毒基因的表达增强，并有助于更好地理解可能有助于降低该害虫对Hal的敏感性的潜在代谢途径。因此，深入了解甘蓝蚜的解毒机制，将为甘蓝蚜的田间管理提供实际指导。图形摘要
The brassica leaf beetle, Phaedon brassicae, is a serious defoliator of cruciferous crops. Halofenozide (Hal), an ecdysone agonist, is a new class of insect growth-regulating insecticide. Our preliminary experiment revealed the outstanding larval toxicity of Hal against P. brassicae. However, the metabolic degradation of this compound in insects remains unclear. In this study, oral administration of Hal at LC10 and LC25 caused severe separation of the cuticle and epidermis, leading to larval molting failure. Sublethal dose exposure also significantly reduced the larval respiration rate as well as their pupation rates and pupal weights. Conversely, the activities of the multifunctional oxidase, carboxylesterase (CarE), and glutathione S-transferase (GST) were significantly enhanced in Hal-treated larvae. Further analysis using RNA sequencing identified 64 differentially expressed detoxifying enzyme genes, including 31 P450s, 13 GSTs, and 20 CarEs. Among the 25 upregulated P450s, 22 genes were clustered into the CYP3 clan, and the other 3 genes belonged to the CYP4 clan. Meanwhile, 3 sigma class GSTs and 7 epsilon class GSTs were dramatically increased, accounting for the majority of the upregulated GSTs. Moreover, 16 of the 18 overexpressed CarEs were clustered into the coleopteran xenobiotic-metabolizing group. These results showed the augmented expression of detoxification genes in P. brassicae after exposed to sublethal dose of Hal, and helped to better understand the potential metabolic pathways that could contribute to the reduced sensitivity to Hal in this pest. Overall, a deep insight into the detoxification mechanisms would provide practical guidance for the field management of P. brassicae. Graphical Abstract