PDGF induces c-myc mRNA expression in MG-63 human osteosarcoma cells but does not stimulate cell replication.
PDGF induces c-myc mRNA expression in MG-63 human osteosarcoma cells but does not stimulate cell replication.
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PDGF 诱导 MG-63 人骨肉瘤细胞中 c-myc mRNA 表达，但不刺激细胞复制。
DOI：
10.1002/jcp.1041320109
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发表时间：
1987
影响因子：
5.6
通讯作者：
Scher,CD
中科院分区：
文献类型：
--
作者：
Womer,RB;Frick,K;Mitchell,CD;Ross,AH;Bishayee,S;Scher,CD
对血小板衍生生长因子（PDGF）调节MG-63人骨肉瘤细胞系（既不表达c-sismRNA也不分泌PDGF类似物）的生长反应进行了表征。Scatchard分析表明，MG-63细胞每个细胞有23，000个受体，kd为5 × 10− 11 M。当32 P-正磷酸盐标记的细胞在4°C下用PDGF处理3 h时，受体以PDGF浓度依赖性方式磷酸化。磷酸化受体在使用针对磷酸酪氨酸的固相单克隆抗体分离32 P标记受体后，通过自显影和凝胶电泳鉴定。受体与抗体的结合被5 mM磷酸苯酯抑制，进一步表明PDGF刺激酪氨酸特异性受体自磷酸化。此外，在北方凝胶上分析，在37°C下用PDGF处理MG-63细胞3小时诱导c-mycmRNA积累增加7.5倍。然而，MG-63细胞在补充有血清（含有PDGF）或血浆（不含）的培养基中生长同样良好。此外，PDGF不刺激生长停滞MG-63细胞的DNA合成，也不增强生长调节素C调节的DNA合成。因此，MG-63细胞是一种天然存在的细胞变体，其中PDGF刺激c-myc表达，但不调节有丝分裂。
The platelet‐derived growth factor (PDGF) modulated growth response of the MG‐63 human osteosarcoma cell line, which neither expresses c‐sismRNA nor secretes a PDGF analogue, was characterized. Scatchard analysis demonstrated that the MG‐63 cells have 23,000 receptors per cell with a kd of 5 × 10−11M. The receptor became phosphorylated, in a PDGF concentration‐dependent manner, when32P‐orthophosphate‐labeled cells were treated with PDGF for 3 h at 4°C. The phosphorylated receptor was identified by autora‐diography and gel electrophoresis after isolation of the32P‐labeled receptor using a solid‐phase monoclonal antibody directed against phosphotyrosine. Binding of the receptor to the antibody was inhibited by 5 mM phenyl phosphate, further suggesting that PDGF stimulated tyrosine‐specific receptor autophosphorylation. In addition, treatment of MG‐63 cells with PDGF for 3 h at 37°C induced a 7.5‐fold increase in c‐mycmRNA accumulation as analyzed on Northern gels. However, MG‐63 cells grew equally well in either serum‐(which contains PDGF) or plasma‐(which does not) supplemented medium. Furthermore, PDGF did not stimulate DNA synthesis in growth arrested MG‐63 cells, nor did it potentiate DNA synthesis modulated by somatomedin C. Thus MG‐63 cells are a naturally occurring cell variant in which PDGF stimulates c‐mycexpression but does not modulate mitogenesis.