Genetic variability among archival cultures of Salmonella typhimurium
Genetic variability among archival cultures of Salmonella typhimurium
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DOI：
10.1111/j.1574-6968.2001.tb10677.x
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发表时间：
2001-05-30
影响因子：
2.1
通讯作者：
Eisenstark, A
中科院分区：
文献类型：
--
作者：
Edwards, K;Linetsky, I;Eisenstark, A
在我们的实验室中，超过10,000个鼠伤寒沙门氏菌LT2培养物在琼脂刺瓶中密封了33-46年，为进化和突变研究提供了机会。在检测的77个小瓶中，每个小瓶中恢复了10(3)-10(2)个菌落形成单位（不到原始种群的0.01%），即使经过数十年的未受干扰的储存。在这些人群中观察到相当大的遗传变异。三个遗传变量，染色体片段大小（脉冲场凝胶电泳测定），从营养性营养不良到原生营养不良的广泛突变逆转，以及蛋白质含量差异（十二烷基硫酸钠聚丙烯酰胺凝胶电泳测定），进行了测量。(C) 2001年欧洲微生物学会联合会。Elsevier Science B.V.版权所有。
The existence in our laboratory of over 10 000 Sulmonella typhimurium LT2 cultures sealed in agar stab vials for 33-46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 10(3)-10(2) colony forming units per vial were recovered (less than 0.01% of the original population) even after decades of undisturbed storage. Considerable genetic variability was observed in these populations. Three genetic variables, chromosome fragment size as determined by pulsed-field gel electrophoresis, extensive mutational reversions from nutritional auxotrophy to prototrophy, and differences in protein content as assayed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, were measured. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.