Identification and characterization of 2 types of erythroid progenitors that express GATA-1 at distinct levels
Identification and characterization of 2 types of erythroid progenitors that express GATA-1 at distinct levels
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DOI：
10.1182/blood-2003-04-1154
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发表时间：
2003-11-15
期刊：
影响因子：
20.3
通讯作者：
Yamamoto, M
中科院分区：
文献类型：
--
作者：
Suzuki, N;Suwabe, N;Yamamoto, M
转录因子加塔-1对红系细胞的发育至关重要。为了确定严格控制加塔-1的表达水平是否是获得适当的红细胞生成所必需的，我们建立了在加塔-1基因造血调控结构域控制下表达绿色荧光蛋白（GFP）的转基因小鼠品系。我们利用转基因小鼠检测了加塔-1的表达水平，并在骨髓中发现了2个GFP阳性的造血祖细胞组分。一种是GFP（高）级分，主要含有CFU-E和共表达转铁蛋白受体的原成红细胞，而另一种是GFP（低）/转铁蛋白受体阴性级分，含有BFU-E。由于绿色荧光的强度与加塔-1的表达水平很好地相关，这些结果表明加塔-1在红系集落形成单位（CFU-E）中高度表达，但在红系爆发形成单位（BFU-E）中低表达，表明加塔-1的增量表达是红系祖细胞形成所必需的。我们还检查了转基因小鼠脾脏和胎肝中的GFP阳性组分，并分别鉴定了含有BFU-E和CFU-E的组分。本研究还建立了一种从小鼠造血组织中富集CFU-E和BFU-E的有效方法。(C)2003年，美国血液学会。
Transcription factor GATA-1 is essential for the development of the erythroid lineage. To ascertain whether strict control of GATA-1 expression level is necessary for achieving proper erythropoiesis, we established transgenic mouse lines expressing green fluorescent protein (GFP) under the control of the GATA-1 gene hematopoietic regulatory domain. We examined the GATA-1 expression level by exploiting the transgenic mice and found 2 GFP-positive hematopoietic progenitor fractions in the bone marrow. One is the GFP(high) fraction containing mainly CFU-E and proerythroblasts, which coexpress transferrin receptor, while the other is the GFP(low)/transferrin receptor-negative fraction containing BFU-E. Since the intensity of green fluorescence correlates well with the expression level of GATA-1, these results indicate that GATA-1 is highly expressed in erythroid colony-forming unit (CFU-E) but low in erythroid burst-forming unit (BFU-E), suggesting that the incremental expression of GATA-1 is required for the formation of erythroid progenitors. We also examined GFP-positive fractions in the transgenic mouse spleen and fetal liver and identified fractions containing BFU-E and CFU-E, respectively. This study also presents an efficient method for enriching the CFU-E and BFU-E from mouse hematopoietic tissues. (C) 2003 by The American Society of Hematology.