Age-associated reduction of nuclear protein import in human fibroblasts
Age-associated reduction of nuclear protein import in human fibroblasts
复制标题
DOI：
10.1016/s0006-291x(02)00492-8
复制
发表时间：
2002-06-07
影响因子：
3.1
通讯作者：
Radu, A
中科院分区：
文献类型：
--
作者：
Pujol, G;Söderqvist, H;Radu, A
通过比较年轻、成熟和老年人类捐赠者的成纤维细胞培养，发现核质转运因子核糖体粘附素α2、CAS和RanBP1的蛋白浓度随年龄的增加而降低。Karyopherin 0-1水平不随年龄变化，在捐赠者之间变化很小.运输因子浓度的降低伴随着来自老年捐赠者的成纤维细胞中蛋白质进口率的降低，这可以从穿梭于细胞质和细胞核之间的测试运输底物的细胞内定位的变化中检测到。对老年小鼠器官和组织中相同进口因子浓度的测量显示，肾、肺和脾中的CAS减少。老年进口的减少预计将导致蛋白质活性受损，其功能依赖于及时输入到细胞核中。(C)2002年埃尔塞维尔科学公司(美国)。版权所有。
Age-dependent decreases in the protein concentrations of the nucleocytoplasmic transport factors karyopherin alpha2, CAS, and RanBP1 were found by comparing fibroblast cultures obtained from young, mature, and old human donors. Karyopherin 0 1 levels do not change with age and present very little variation among donors. The decrease in the concentration of transport factors is accompanied by a reduction in the protein import rate in fibroblasts from old donors, as detected by a change in the intracellular localization of a test transport substrate that shuttles between the cytoplasm and the nucleus. Measurements of concentrations of the same import factors in organs and tissues of old mice revealed a decrease of CAS in kidney, lung, and spleen. The import reduction in old age is expected to lead to impaired activity of proteins whose functions depend on timely import into the nuclei. (C) 2002 Elsevier Science (USA). All rights reserved.