Microarray analysis of gene expression in the ovarian cancer cell line HO-8910 with silencing of the ZNF217 gene
Microarray analysis of gene expression in the ovarian cancer cell line HO-8910 with silencing of the ZNF217 gene
复制标题
ZNF217 基因沉默的卵巢癌细胞系 HO-8910 中基因表达的微阵列分析
DOI：
10.3892/mmr_00000183
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发表时间：
2009-09-01
影响因子：
3.4
通讯作者：
Zhong, Mei
中科院分区：
文献类型：
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作者：
Sun, Guiqin;Qin, Jingxia;Zhong, Mei
锌指蛋白217(ZNF217)在肿瘤进展过程中过度表达，称为促进肿瘤细胞永生化。为了研究ZNF217的功能，将ZNF217基因沉默后，用HG-U133 Plus 2.0芯片芯片对卵巢癌细胞株HO-8910进行了整体表达谱分析。使用基因本体论程序对结果进行分析，以研究ZNF217对卵巢癌细胞功能网络的影响。实时定量逆转录聚合酶链式反应(RT-PCR)检测受影响基因的mRNA表达变化。结果表明，ZNF217基因是卵巢癌的关键调节基因，因为ZNF217的沉默导致HO-8910细胞系中164个基因的表达显著下调，与未沉默的对照细胞相比至少下调了8倍。在这些下调的基因中有ALOX15。CD1d、FXYD3、Gas6、KRT4。LIN7B、MMP24、PDZK1、PEX6。PRSS8、SLC2A9、STRN和WFDC2。实时荧光定量RT-PCR检测证实ZNF217基因沉默后表达下调(p
Zinc-finger protein 217 (ZNF217), which is overexpressed during cancer progression, call promote tumor cell immortalization. To examine the function of ZNF217, a global expression profile was carried Out using Affymetrix Gene Chip analysis with HG-U133 Plus 2.0 arrays in the ovarian cancer cell title HO-8910 after silencing of file ZNF217 gene. The results were analyzed using the Gene Ontology program to investigate the functional network affected by ZNF217 in ovarian cancer cells. Changes in the mRNA expression of the affected genes were confirmed by real-time reverse transcriptase-polumerase chain reaction (RT-PCR). The results showed that the ZNF217 gene is a key regulator of ovarian cancer, as the silencing of ZNF217 resulted in significant down-regulation by at least 8-fold of 164 genes in the HO-8910 cell line compared to non-silenced control cells. Among these down-regulated genes were ALOX15. CD1D, FXYD3, GAS6, KRT4. LIN7B, MMP-24, PDZK1, PEX6. PRSS8, SLC2A9, STRN and WFDC2. Down-regulation was confirmed by realtime RT-PCR after file silencing of ZNF217 (p