Conditional induction of ovulation in mice.
Conditional induction of ovulation in mice.
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有条件诱导小鼠排卵。
DOI：
10.1095/biolreprod.104.039164
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发表时间：
2005
影响因子：
3.6
通讯作者：
Miller,WilliamL
中科院分区：
文献类型：
--
作者：
Su,Pei;Wu,JoyceC;Sommer,JeffreyR;Gore,AJesse;Petters,RobertM;Miller,WilliamL
促卵泡激素控制哺乳动物卵巢卵泡的成熟。过量的话，它可以促进排卵（产蛋）。本文报道了一种转基因多西环素激活开关，在小鼠中进行了测试，通过简单喂食多西环素（Dox）产生更多的FSHB亚基（因此更多的FSH）和增加排卵。转基因开关在垂体促性腺激素中选择性表达，旨在增强暴露于Dox时FSH的正常表达，但受体内正常控制FSH产生的所有激素的调节。饲喂最高有效水平的Dox可使雄性FSHB和血清FSH总mRNA增加一半以上，并且Dox处理可使雌性小鼠的正常排卵率增加一倍以上，可达10个生殖周期。低剂量的Dox可使胚胎发育数量增加30%。卵巢结构和功能正常。综上所述，基因开关技术与正常FSH调节相结合可有效促进小鼠排卵。从理论上讲，同样的策略可以用于任何遗传开关来增加任何哺乳动物的排卵（或任何高度保守的生理）。
Follicle-stimulating hormone controls the maturation of mammalian ovarian follicles. In excess, it can increase ovulation (egg production). Reported here is a transgenic doxycycline-activated switch, tested in mice, that produced more FSHB subunit (therefore more FSH) and increased ovulation by the simple feeding of doxycycline (Dox). The transgenic switch was expressed selectively in pituitary gonadotropes and was designed to enhance normal expression of FSH when exposed to Dox, but to be regulated by all the hormones that normally control FSH production in vivo. Feeding maximally effective levels of Dox increased overall mRNA for FSHB and serum FSH by over half in males, and Dox treatment more than doubled the normal ovulation rate of female mice for up to 10 reproductive cycles. Lower levels of Dox increased the number of developing embryos by 30%. Ovarian structure and function appeared normal. In summary, gene switch technology and normal FSH regulation were combined to effectively enhance ovulation in mice. Theoretically, the same strategy can be used with any genetic switch to increase ovulation (or any highly conserved physiology) in any mammal.