Modeling Multispecies Gene Flow Dynamics Reveals the Unique Roles of Different Horizontal Gene Transfer Mechanisms
Modeling Multispecies Gene Flow Dynamics Reveals the Unique Roles of Different Horizontal Gene Transfer Mechanisms
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DOI：
10.3389/fmicb.2018.02978
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发表时间：
2018-12-04
影响因子：
5.2
通讯作者：
Boedicker, James Q.
中科院分区：
文献类型：
--
作者：
Nazarian, Phillip;Tran, Frances;Boedicker, James Q.
不同细菌群体内的水平基因转移通过多种交换机制发生。最确定的基因转移，转导，转化和接合的途径，已被详细描述，揭示了每种机制的优点和局限性。最近，通过细胞外囊泡的种间基因交换已被报道和表征，使得囊泡介导的交换成为基因转移的第四种一般机制。尽管对每种途径都有了解，但尚未探索所有这些机制如何协同作用。在这里，我们开发了一个多物种细菌群落中的基因交换模型，该模型考虑了所有四种基因转移机制的速率和限制。我们的研究结果揭示了每种基因交换机制的独特作用，并强调了多种途径共同作用是不同细菌群体中广泛基因交换所必需的。
Horizontal gene transfer within diverse bacterial populations occurs through multiple mechanisms of exchange. The most established routes of gene transfer, transduction, transformation, and conjugation, have been characterized in detail, revealing the advantages and limitations of each mechanism. More recently, interspecies gene exchange via extracellular vesicles has been reported and characterized, making vesicle-mediated exchange a fourth, general mechanism of gene transfer. Despite an understanding of each individual pathway, how all of these mechanisms act in concert has not been explored. Here we develop a model of gene exchange in a multispecies bacterial community that takes into account the rates and limitations of all four gene transfer mechanisms. Our results reveal unique roles for each gene exchange mechanism, and highlight how multiple pathways working together are required for widespread gene exchange within diverse bacterial populations.