Diagnostic and predictive significance of serum microRNA-7 in esophageal squamous cell carcinoma
Diagnostic and predictive significance of serum microRNA-7 in esophageal squamous cell carcinoma
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DOI：
10.3892/or.2015.4499
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发表时间：
2016-03-01
期刊：
影响因子：
4.2
通讯作者：
Zhang, Peiliang
中科院分区：
文献类型：
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作者：
Dong, Wei;Li, Baosheng;Zhang, Peiliang
据报道，MicroRNA-7通过多种肿瘤中的多种信号通路参与肿瘤的发生和进展。然而，其作为食管鳞状细胞癌（ESCC）的血清诊断因子和预测生物标志物的潜力尚未得到研究。血清样本采集自 105 名经病理证实的 ESCC 患者和 30 名年龄和性别匹配的健康对照。所有患者均接受同步放化疗（CRT）治疗。实时聚合酶链反应测定血清miR-7表达水平。对放射敏感组和放射抗性组以及健康志愿者的数据进行比较，以阐明 miR-7 表达的诊断和预测价值。最后，利用体外实验阐明miR-7的机制。在本研究中，我们发现食管鳞癌患者的血清miR-7水平比健康受试者低4.74倍，表明血清miR-7表达可能是一个很好的诊断因素。这些有反应患者的血清 miR-7 表达水平比无反应患者高 2.34 倍，表明它是预测 ESCC 患者对同步放化疗治疗反应的有价值的生物标志物。我们还发现 miR-7 水平与肿瘤长度和淋巴结转移状态密切相关（P
MicroRNA-7 has been reported to participate in tumorigenesis and progression by several signaling pathways in various tumors. However, its potential as a serum diagnostic factor and predictive biomarker for esophageal squamous cell carcinoma (ESCC) has not been studied. Serum samples were collected from 105 pathologically proven ESCC patients and 30 age-and gender-matched healthy controls. All patients were treated with concurrent chemoradiotherapy (CRT). Real-time polymerase chain reaction was carried out to measure the serum miR-7 expression level. The data were compared among radio-sensitive and radio-resistant groups, and healthy volunteers to elucidate the diagnostic and predictive value of miR-7 expression. Finally, in vitro experiments are used to clarify the mechanisms of the miR-7. In the present study, we found that the serum miR-7 level of ESCC patients was 4.74-fold lower as compared with healthy subjects, indicating that serum miR-7 expression could be an excellent diagnostic factor. The serum miR-7 expression level for these responsive patients was 2.34-fold higher than that for non-responsive patients, indicating it as a valuable biomarker for predicting treatment response of ESCC patients to concurrent chemoradiation treatment. We also found that miR-7 levels are strongly correlated with tumor length and the status of lymph node metastasis (P